Effect of prior exercise on glucose metabolism in trained men

Adam J. Rose1, Kirsten Howlett1, Douglas S. King2, and Mark Hargreaves1

1 School of Health Sciences, Deakin University, Burwood, 3125 Victoria, Australia; and 2 Department of Health and Human Performance, Iowa State University, Ames, Iowa 50011


    ABSTRACT
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Several studies have demonstrated that oral glucose tolerance is impaired in the immediate postexercise period. A double-tracer technique was used to examine glucose kinetics during a 2-h oral glucose (75 g) tolerance test (OGTT) 30 min after exercise (Ex, 55 min at 71 ± 2% of peak O2 uptake) and 24 h after exercise (Rest) in endurance-trained men. The area under the plasma glucose curve was 71% greater in Ex than in Rest (P = 0.01). The higher glucose response occurred even though whole body rate of glucose disappearance was 24% higher after exercise (P = 0.04, main effect). Whole body rate of glucose appearance was 25% higher after exercise (P = 0.03, main effect). There were no differences in total (2 h) endogenous glucose appearance (RaE) or the magnitude of suppression of RaE, although RaE was higher from 15 to 30 min during the OGTT in Ex. However, the cumulative appearance of oral glucose was 30% higher in Ex (P = 0.03, main effect). There were no differences in glucose clearance rate or plasma insulin responses between the two conditions. These results suggest that adaptations in splanchnic tissues by prior exercise facilitate greater glucose output from the splanchnic region after glucose ingestion, resulting in a greater glycemic response and, consequently, a greater rate of whole body glucose uptake.

glucose homeostasis; glucose kinetics; physical activity


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

THE GLYCEMIC RESPONSE to ingested carbohydrate is greater in the period immediately after exercise (2, 3, 15, 20), whereas the insulinemic response may be greater (15), not different (2), or lower (20, 21). Although these results indicate a relative reduction in whole body insulin action in the period immediately after exercise, the physiological bases of this response remain unclear. King et al. (15) suggested that this apparent insulin resistance may be due to elevations in hormones and metabolites after exercise, which may act to impede peripheral glucose uptake, particularly in skeletal muscle. Indeed, others have observed that nonexercised muscle is insulin resistant after exercise (6). However, other studies have revealed that net glucose output from the splanchnic (liver/gut) tissues during a gut glucose load is greater in the period immediately after exercise than in the rested state (12, 16). Therefore, the roles of peripheral uptake and splanchnic output of glucose in altering glucose tolerance during the period immediately after exercise are not clear.

The aim of the present study was to examine the effect of prior exercise on three primary determinants of oral glucose tolerance. Our approach was to use the double-tracer technique to determine the rate of appearance of exogenous glucose (RaE), endogenous glucose production, and whole body glucose uptake after exercise. To distinguish between the transient effects of acute exercise on glucose tolerance and insulin action in humans who habitually exercise, we chose to examine glucose kinetics in endurance-trained individuals.


    METHODS
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

Subjects. The study group consisted of six healthy, endurance-trained male volunteers (29.2 ± 2.0 yr, 72.9 ± 3.7 kg, 1.77 ± 0.03 m). All subjects had been exercising regularly (6.2 ± 0.4 days/wk, 1.1 ± 0.2 h/day) for at least the prior 6 mo, and physical training frequently involved cycling or running. Written information about the purpose, nature, and potential risks relating to experimental procedures was given to the subjects before they provided consent to participate. The protocol was reviewed and approved by the Deakin University Human Ethics Committee.

Preexperimental protocol. Subjects were instructed to maintain their normal exercise regimen throughout the entire trial period and refrain from exercise (other than that required for normal living), smoking, and the consumption of alcohol or caffeine for 24 h before testing. Initially, participants completed an incremental cycle exercise test to volitional exhaustion to determine peak rate of pulmonary O2 uptake (VO2 peak). VO2 peak and peak power output were 65.4 ± 2.8 ml · kg-1 · min-1 and 5.91 ± 0.24 W/kg, respectively. This test and the subsequent exercise session were conducted on an electromagnetically braked cycle ergometer (Lode, Groningen, The Netherlands). During exercise, expired air was analyzed using a metabolic cart (Gould 2900 Metabolic System), and heart rate (HR) was recorded (Polar Electro). Before each use, the flowmeter and chemical sensors of the metabolic cart were calibrated using a syringe of known volume and gases of known composition.

Subjects were instructed to complete an exercise bout (45-75 min) at 70-80% of their peak HR 24 h before each trial. In addition, they were provided with a food package for consumption containing ~14 MJ (~80% carbohydrate) on the day before each trial, and they were instructed to consume 5 ml of water per kilogram of body mass on waking in the morning of the trial to ensure adequate hydration. Subjects were studied after a 12-h overnight fast.

Experimental protocol. Subjects presented to the laboratory for two separate trials: an exercise (Ex) and a 24-h postexercise (Rest) trial. Before the commencement of each trial, a Teflon catheter was inserted into a forearm vein of one arm for blood sampling and the contralateral arm for infusion. The catheter for blood sampling was kept patent throughout each trial by periodic injection of 0.9% saline containing a small amount of heparin. Immediately after a blood sample was obtained, a priming dose of [6,6-2H]glucose (3.3 mmol; Cambridge Isotope Laboratories, Cambridge, MA) was administered, and a continuous infusion (44.0 ± 0.4 µmol/min) of diluted isotope was started and maintained throughout the duration of a trial. Each trial had an initial 2-h infusion period, during which subjects rested in the seated position to allow tracer equilibration.

In Rest, an oral glucose tolerance test (OGTT) was performed at the end of the 2-h rest period. In Ex, after the 2-h rest period, subjects exercised on a cycle ergometer at a work rate approximating 70% VO2 peak for 60 min (which included a 5-min warm-up at ~50% VO2 peak). The OGTT was started 30 min after completion of the exercise bout. During exercise, expired air was collected and analyzed for O2 consumption and HR was recorded at 15-min intervals. The 2-h OGTT commenced with the ingestion of 75 g of glucose tolerance test solution (300 ml) to which 60 µCi of [3-3H]glucose (Du Pont Biotechnology Systems, Wilmington, DE) had been added. No food or liquid was consumed throughout either of the trials other than the glucose drink. The resting trial was staggered such that the ingestion of the glucose drink in the two trials occurred at approximately the same time of day to account for diurnal variation in hormonal and metabolic responses. Trials were counterbalanced between subjects.

Venous blood was sampled at 5-min intervals during the last 10 min of the exercise bout and 10 min before each OGTT to determine baseline levels. During each OGTT, blood was sampled at 15-min intervals for 2 h after ingestion of the glucose load. Blood samples for glucose, isotopically labeled glucose, and lactate were taken at each sampling point for both trials. Samples for nonesterified fatty acids (NEFA) and insulin were collected at 30-min intervals during the OGTT. Samples for hematocrit (Hct) were collected immediately before the exercise bout in Ex and at 0, 60, and 120 min of the OGTT for both trials.

Analytic techniques. Samples for glucose, lactate, and insulin were collected in lithium heparin tubes, and NEFA samples were collected in tubes containing 30 µl of EGTA and reduced glutathione. All blood samples were immediately spun, and the plasma was kept on ice for the remainder of each experiment. Thereafter, samples were stored at -20°C until analysis. Whole blood was collected in lithium heparin tubes and placed on ice for subsequent analysis of Hct.

Plasma glucose and lactate concentrations were measured using an automated analyzer (model EML 105, Radiometer). Plasma insulin concentrations were determined by RIA (Phadeseph, Pharmacia and Upjohn). Plasma NEFA concentrations were determined using an enzymatic-colorimetric method (NEFA C test kit, Wako Chemicals). Hct was determined by the microcapillary centrifugation method. Plasma, drink, and infusate [3H]glucose and [2H]glucose were analyzed using a modified method as described in detail previously (18). Briefly, plasma aliquots were deproteinized, and the protein-free supernatant was evaporated to remove labeled water. To determine [3H]glucose radioactivity, the dried product was reconstituted with distilled water, scintillant was added, and the samples were counted by a liquid scintillation counter (model LS 3801, Beckman). To determine [2H]glucose enrichment, a derivatization solution (2:3 pyridine-acetic anhydride) was added to the dried product and measured by gas chromatography-mass spectrophotometry (5890 series 2 gas chromatograph and 5971 mass spectrophotometer detector, Hewlett-Packard, Avondale, PA).

Calculations and statistics. The integrated areas under the plasma glucose and insulin curves were calculated using the trapezoidal model (15). Rates of plasma glucose appearance (Ra) and disappearance (Rd) were calculated using a modified monocompartmental non-steady-state model (26) with the assumption that the apparent glucose space was 25% body weight and pool fraction was 0.65. The metabolic clearance rate (MCR) of glucose was calculated by dividing Rd by the corresponding plasma glucose concentration. The Ra of oral glucose (RaO) and endogenous glucose (RaE) were calculated using the method of Steele et al. (25) as used with stable glucose isotopes (23). To calculate an insulin sensitivity index, the glucose Rd values at 30, 60, and 90 min were divided by the corresponding plasma insulin concentration and the mean of these values was taken. The data from the two trials were compared by ANOVA for repeated measures. Specific differences were located with the Student-Newman-Keuls post hoc test. A paired t-test was used to locate differences in areas under the response curves between the two conditions. Values are means ± SE.


    RESULTS
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

In Ex, subjects exercised at a work rate requiring 71 ± 2% VO2 peak for 55 min on a cycle ergometer, which corresponded to 57 ± 1% peak power output and 82 ± 2% peak HR. Venous plasma lactate was 3.9 ± 0.8 mmol/l during the final 10 min of exercise.

Plasma glucose was not significantly different before glucose ingestion between Rest and Ex (Fig. 1A). The integrated area under the plasma glucose curve was 71% greater in Ex than in Rest (P = 0.01; Fig. 1A). Whole body glucose Ra increased to a greater extent after oral glucose in Ex (P = 0.03, main effect). The total Ra during the 2 h after glucose ingestion was 25% greater in Ex (P = 0.02; Fig. 1B). Whole body glucose Rd rose to greater levels after glucose ingestion in Ex (P = 0.04, main effect), with total glucose uptake during the 2 h after glucose ingestion being 24% greater (P = 0.03; Fig. 1C). The MCRs of glucose were not different between Rest and Ex before or after glucose ingestion (Fig. 2A). Although RaE was 8.6 µmol · kg-1 · min-1 greater in Ex than in Rest in the preingestion period, this difference did not reach statistical significance (P = 0.22). However, RaE was significantly higher from 15 to 30 min during the OGTT in Ex than in Rest (Fig. 2B). Despite this early difference, there were no differences in the cumulative RaE (Fig. 2B) or the magnitude of suppression of RaE during the OGTT between Rest (43 ± 4%) and Ex (48 ± 11%). RaO was significantly greater during the OGTT in Ex (P = 0.03, main effect), with the cumulative RaO being 30% greater in Ex (P = 0.03, Fig. 2C). Thus the greater increase in whole body Ra when glucose was ingested after exercise was due, at least in part, to a greater RaO.


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Fig. 1.   Plasma glucose and whole body glucose rate of appearance (Ra) and disappearance (Rd) during a 75-g oral glucose tolerance test (OGTT) immediately after exercise (open circle , Ex) and 24 h after exercise (, Rest). Integrated areas under the response curves during the 2-h OGTT are shown at right. Values are means ± SE (n = 6). *Significantly different from Rest, P < 0.05.



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Fig. 2.   Metabolic clearance rate (MCR), endogenous glucose appearance (RaE), and appearance of oral glucose (RaO) during a 75-g OGTT immediately after exercise (open circle , Ex) and 24 h after exercise (, Rest). Integrated areas under the response curves during the 2-h OGTT are shown at right. Values are means ± SE (n = 6). *Significantly different from Rest, P < 0.05.

Plasma insulin was not different between Ex and Rest before and during the OGTT (Table 1). The integrated areas under the insulin curves were also not different between Ex and Rest (13,320 ± 1,751 and 12,759 ± 1,810 pmol · l-1 · min, respectively). There was a significantly higher insulin sensitivity index (glucose Rd divide  insulin concentration) in Ex than in Rest (0.49 ± 0.07 vs. 0.35 ± 0.04 µmol · kg-1 · min-1 · pmol-1 · l, P = 0.02), despite no difference in glucose MCR. Plasma NEFA concentration was significantly higher before glucose ingestion in Ex but was reduced to similar concentrations in Ex and Rest during the OGTT (Table 1). There were no differences in the plasma lactate response before and during the OGTT between Ex and Rest (Table 1). There were no differences in Hct before and during the OGTT between Ex and Rest (data not shown).

                              
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Table 1.   Plasma insulin, NEFA, and lactate responses during a 75-g OGTT immediately after exercise and 24 h after exercise


    DISCUSSION
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

The results demonstrate an exaggerated glycemic response to oral glucose ingested in the period immediately (30 min) after exercise compared with that ingested 24 h after exercise. This finding is consistent with several similar studies in untrained (2) and moderately trained (15) middle-aged people as well as endurance-trained men (20). This greater glycemic response could be due to an effect of prior exercise to increase whole body glucose Ra, decrease whole body glucose uptake, or a combination of both. In the present study, whole body glucose Ra was 25% higher when glucose was ingested after exercise. Similarly, Maehlum et al. (16) observed a 50-300% greater net glucose output from splanchnic tissues over a 3-h period after ingestion of 100 g of glucose subsequent to exhaustive exercise in untrained men. Furthermore, a greater increase in glucose Ra in the initial period of intraduodenal glucose infusion has been observed in exercised dogs (12). Thus, when glucose is ingested in the early postexercise period, the heightened plasma glucose response is mediated, at least in part, by a higher whole body glucose Ra.

Net glucose output from the splanchnic tissues includes both the net rate of liver glucose output and the rate at which glucose is absorbed from the gut. In the present study, the rate at which oral glucose appeared in the peripheral venous circulation was 30% higher during the 2-h OGTT after exercise. This result is consistent with findings of Hamilton et al. (12), which demonstrate that glucose output from the gut is elevated in exercised dogs when measured by tracer and arterial-venous difference techniques. The higher RaE could be due to a relative increase in intestinal absorption rate or a reduction in glucose extraction by the liver during first pass from the portal circulation.

Maehlum et al. (16) observed 15-35% greater rate of absolute splanchnic blood flow (SBF) after oral glucose in exercised than in rested men. Because mesenteric blood flow is positively related to intestinal glucose absorption (28), a higher SBF may be a contributing factor to the higher RaO observed after exercise in this study. In contrast, the increase in gut glucose absorption rate after exercise observed by Hamilton et al. (12) occurred despite any significant changes in SBF, suggesting that intestinal permeability to glucose is higher after exercise. Although the mechanism for increased intestinal permeability after exercise is not clear, carrier-mediated intestinal glucose absorption may be decreased during exercise in trained men (27), possibly attributable to the reduction in SBF during high-intensity activity. To our knowledge, there are no studies that have examined carrier-mediated glucose transport in the postexercise state. Interestingly, however, there is evidence that paracellular permeability is increased after high-intensity (80% VO2 peak) running exercise (22). Regardless of the mechanism, the present results are compatible with a previously observed finding that the gut glucose output in response to intraduodenal glucose infusion is augmented after exercise in dogs (12).

The elevated net splanchnic glucose output observed by Maehlum et al. (16) persisted when measured 12 h after exercise, during which the subjects were starved. In addition, it has been demonstrated that gut glucose output is greater during an identical intestinal glucose load in 42-h- vs. 18-h-fasted dogs (8). Thus whether this effect is the result of exercise per se or simply the result of inducing a greater negative energy balance is unclear.

Burke et al. (3) suggested that the greater splanchnic glucose output observed after exercise by Maehlum et al. (16) is related to a selective hepatic insulin resistance. Indeed, there is indirect evidence that prior exercise may reduce the absolute rate of hepatic glucose uptake during a gut glucose load. Hamilton et al. (12) observed no change in net hepatic glucose uptake and a greater suppression of endogenous glucose production during intraduodenal infusion in exercised dogs. If the contribution of the kidneys to endogenous glucose production is assumed to be minor (4), then this finding suggests that the absolute rate of hepatic glucose uptake was reduced after exercise. In contrast to this indirect finding, studies in diabetic humans (14) and in animal models (8, 17) indicate that prior exercise augments hepatic glucose uptake. In particular, a study in dogs by Galassetti et al. (8) revealed that when the primary determinants of net hepatic glucose uptake (i.e., hepatic arterial-portal venous glucose gradient, glycemia, insulin, and glucagon) were controlled, prior exercise augments net hepatic glucose uptake. Thus it is unclear whether the absolute rate of hepatic glucose uptake was reduced in the present study. Furthermore, because the extraction of glucose by the liver on first pass from the portal circulation contributes only ~5% to total disposal (7), any reduction in hepatic glucose disposal probably cannot quantitatively contribute to the 30% greater increase in RaO observed after exercise.

A reduced suppression of RaE would also contribute to the greater rise in total Ra after glucose ingestion in the postexercise period. In the present study, RaE was significantly higher from 15 to 30 min after oral glucose in Ex. The reason behind this response is unclear but may be related to elevated glucagon after exercise (12). Although exercise reduces hepatic glycogen stores (13), the liver remains a net producer of glucose in the postabsorptive state after exercise (1). In the present study, we observed no significant difference in the cumulative RaE and magnitude of suppression of RaE during the OGTT. This finding is consistent with a study using a graded, hyperinsulinemic-euglycemic clamp technique, which demonstrated no significant difference in the sensitivity or responsiveness of insulin to suppress RaE in the period immediately after exercise compared with 15 h after exercise in trained men (19).

The greater Ra during the OGTT after exercise would, via mass action, increase whole body glucose uptake. There was a 24% greater increase in whole body glucose Rd during the 2-h OGTT after exercise, which is likely to be mainly attributable to greater glucose disposal in previously active muscle (12). King et al. (15) demonstrated an apparent insulin resistance in moderately trained middle-aged people immediately after exercise and suggested that elevations in counterregulatory hormones and metabolites may impede glucose Rd in the immediate postexercise state. Indeed, elevated levels of plasma NEFA and triglycerides produce muscle insulin resistance and impair glycogen synthesis in the postexercise state (5). However, in the present study, although the plasma NEFA were higher after exercise immediately before the OGTT, by 30 min NEFA had returned to concentrations that were not different from those observed in the rested condition during the OGTT. The rapid decrease in plasma NEFA in the present study may be related to the high level of fitness of the subjects in this study, since endurance training augments the suppression of adipocyte lipolysis by insulin (24).

Because the glucose MCRs and plasma insulin responses were not different between exercised and rested conditions, it is unlikely that whole body insulin sensitivity was altered after exercise. Similarly, Mikines et al. (19) demonstrated no difference in whole body insulin action in the period immediately after exercise vs. 15 h after exercise in trained men. However, a negative hepatic arterial-portal venous glucose gradient, which is not present during a clamp situation, can impede peripheral glucose uptake in resting dogs (11). Interestingly, prior exercise eliminates the inhibitory effect of the portal signal on peripheral glucose disposal, particularly in contracted skeletal muscle (10). Thus it is conceivable that prior exercise alters the balance between splanchnic and peripheral glucose disposal after glucose ingestion, perhaps for preferential synthesis of glycogen in contracted skeletal muscle. However, there was no difference in the balance between hepatic and peripheral glucose disposal between exercised and rested dogs during portal glucose infusion (10). Conversely, in the present study, a higher intestinal absorption rate after exercise may create an effect whereby the glucose load reaching the liver saturates the rate at which the liver can take up glucose, thereby facilitating enhanced splanchnic glucose escape. Thus, as demonstrated by the present study and previous studies (12, 16), adaptations to splanchnic tissues by prior exercise facilitate the delivery of ingested glucose to the systemic circulation, thereby enhancing peripheral glucose disposal.

Some authors have attributed the impaired glucose tolerance after exercise to a reduced insulin response (20, 21), whereas others have demonstrated no difference (2) or higher (15) insulin responses after exercise. The results of the present study also show a lower insulin response relative to the increase in plasma glucose; nevertheless, there was a greater increase in glucose Rd, with no difference in glucose clearance, and a higher insulin sensitivity index when glucose was ingested after exercise. Thus the present data suggest that it is unlikely that a lower insulin response contributes to the apparent glucose intolerance after exercise.

In summary, the greater glycemic response when glucose is ingested after exercise is mediated by a greater Ra, the majority of which is due to a greater RaO. This heightened RaO is probably due to exercise effects on splanchnic tissues to increase intestinal absorption and/or reduce hepatic uptake of glucose. Because there were no differences in plasma insulin or glucose clearance rate between exercised and rested states, the greater whole body glucose uptake appears to be mediated by the exaggerated glycemic response to oral glucose after exercise. This study demonstrates that, along with adaptations within active muscle itself, adaptations in splanchnic tissues by prior exercise may facilitate whole body glucose disposal and the resynthesis of muscle glycogen.


    ACKNOWLEDGEMENTS

The authors thank Dr. Andrew Garnham for medical assistance, Andrew Sanigorski (School of Health Sciences, Deakin University) and Dom Caridi (Dept. of Chemistry and Biology, Victoria University of Technology) for excellent technical assistance, and Prof. Joe Proietto for helpful advice in preparation of the manuscript.


    FOOTNOTES

This research was partly funded by a grant to D. S. King from Experimental and Applied Sciences (Golden, CO).

Address for correspondence: M. Hargreaves, School of Health Sciences, Deakin University, Burwood, 3125 Victoria, Australia (E-mail: mharg{at}deakin.edu.au).

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 14 November 2000; accepted in final form 17 May 2001.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
METHODS
RESULTS
DISCUSSION
REFERENCES

1.   Ahlborg, G, and Felig P. Lactate and glucose exchange across the forearm, legs, and splanchnic bed during and after prolonged leg exercise. J Clin Invest 69: 45-54, 1982[ISI][Medline].

2.   Bonen, A, Ball-Burnett M, and Russel C. Glucose tolerance is improved after low- and high-intensity exercise in middle-age men and women. Can J Appl Physiol 23: 583-593, 1998[ISI][Medline].

3.   Burke, LM, Collier GR, and Hargreaves M. Muscle glycogen storage after prolonged exercise: effect of glycemic index of carbohydrate feedings. J Appl Physiol 75: 1019-1023, 1993[Abstract].

4.   Cersosimo, E, Garlick P, and Ferretti J. Regulation of splanchnic and renal substrate supply by insulin in humans. Metabolism 49: 676-683, 2000[ISI][Medline].

5.   Delmas-Beauvieux, M, Quesson B, Thiaudiere E, Gallis J, Canioni P, and Gin H. 13C nuclear magnetic resonance study of glycogen resynthesis in muscle after glycogen-depleting exercise in healthy men receiving an infusion of lipid emulsion. Diabetes 48: 327-333, 1999[Abstract/Free Full Text].

6.   Devlin, JT, Barlow J, and Horton ES. Whole body and regional fuel metabolism during early postexercise recovery. Am J Physiol Endocrinol Metab 256: E167-E172, 1989[Abstract/Free Full Text].

7.   Ferrannini, E, Bjorkman O, Reichard GA, Jr, Pilo A, Olsson M, Wahen J, and DeFronzo RA. The disposal of an oral glucose load in healthy subjects: a quantitative study. Diabetes 34: 580-588, 1985[Abstract].

8.   Galassetti, P, Coker RH, Lacy DB, Cherrington AD, and Wasserman DH. Prior exercise increases net hepatic glucose uptake during a glucose load. Am J Physiol Endocrinol Metab 276: E1022-E1029, 1999[Abstract/Free Full Text].

9.   Galassetti, P, Hamilton KS, Gibbons FK, Bracy DP, Lacy DB, Cherrington AD, and Wasserman DH. Effect of fast duration on the disposition of an intraduodenal glucose load in conscious dogs. Am J Physiol Endocrinol Metab 276: E543-E552, 1999[Abstract/Free Full Text].

10.   Galassetti, P, Koyama Y, Coker RH, Lacy DB, Cherrington AD, and Wasserman DH. Role of a negative arterial-portal venous glucose gradient in the postexercise state. Am J Physiol Endocrinol Metab 277: E1038-E1045, 1999[Abstract/Free Full Text].

11.   Galassetti, P, Shiota M, Zinker BA, Wasserman DH, and Cherrington AD. A negative arterial-portal venous glucose gradient decreases skeletal muscle glucose uptake. Am J Physiol Endocrinol Metab 275: E101-E111, 1998[Abstract/Free Full Text].

12.   Hamilton, KS, Gibbons FK, Bracy DP, Lacy DB, Cherrington AD, and Wasserman DH. Effect of prior exercise on the partitioning of an intestinal glucose load between splanchnic bed and skeletal muscle. J Clin Invest 98: 125-135, 1996[Abstract/Free Full Text].

13.   Hultman, E, and Nilsson LH. Liver glycogen in man: effect of different diets and muscular exercise. Adv Exp Med Biol 11: 143-151, 1971.

14.   Kawamori, R, Kubota M, Ikeda M, Matsuhisa M, Kubota M, Morishima T, and Kamada T. Quantitative determination of hepatic glucose uptake using an innovative approach: effect of strict glycemic regulation and exercise in diabetic subjects. J Nutr Sci Vitaminol (Tokyo) 37: S35-S42, 1991[ISI][Medline].

15.   King, DS, Baldus PJ, Sharp RL, Kesl LD, Feltmeyer TL, and Riddle MS. Time course for exercise-induced alterations in insulin action and glucose tolerance in middle-aged people. J Appl Physiol 78: 17-22, 1995[Abstract/Free Full Text].

16.   Maehlum, S, Felig P, and Wahen J. Splanchnic glucose and muscle glycogen metabolism after glucose feeding during postexercise recovery. Am J Physiol Endocrinol Metab Gastrointest Physiol 235: E255-E260, 1978[Abstract/Free Full Text].

17.   Matsuhisa, M, Nishizawa H, Ikeda M, Kubota M, Arai S, Kawamori R, and Yamasaki Y. Prior muscular contraction enhances disposal of glucose analog in the liver and muscle. Metabolism 47: 44-49, 1998[ISI][Medline].

18.   McConell, G, Fabris S, Proietto J, and Hargreaves M. Effect of carbohydrate ingestion on glucose kinetics during exercise. J Appl Physiol 77: 1537-1541, 1994[Abstract/Free Full Text].

19.   Mikines, KJ, Sonne B, Tronier B, and Galbo H. Effects of acute exercise and detraining on insulin action in trained men. J Appl Physiol 66: 704-711, 1989[Abstract/Free Full Text].

20.   O'Connor, AM, Johnston CF, O'Gorman D, Kirwan JP, Buchanan KD, and Alberti KGMM Glucose intolerance following endurance exercise (Abstract). Regul Pept 51: 289, 1994.

21.   O'Gorman, DJ, Del Aguila LF, Williamson DL, Krishnan RK, and Kirwan JP. Insulin and exercise differentially regulate PI3-kinase and glycogen synthase in human skeletal muscle. J Appl Physiol 89: 1412-1419, 2000[Abstract/Free Full Text].

22.   Pals, KL, Chang R, Ryan AJ, and Gisolfi CV. Effect of running intensity on intestinal permeability. J Appl Physiol 82: 571-576, 1997[Abstract/Free Full Text].

23.   Proietto, J. Estimation of glucose kinetics following an oral glucose load: methods and applications. Horm Metab Res Suppl 24: 25-30, 1990[Medline].

24.   Stallnecht, B, Larsen JJ, Mikines KJ, Simonsen L, Bülow J, and Galbo H. Effect of training on insulin sensitivity and lipolysis in human adipose tissue. Am J Physiol Endocrinol Metab 279: E376-E385, 2000[Abstract/Free Full Text].

25.   Steele, R, Bjerknes C, Rathgeb I, and Altszuler N. Glucose uptake and production during the oral glucose tolerance test. Diabetes 17: 415-421, 1968[ISI][Medline].

26.   Steele, R, Wall JS, DeBodo RC, and Altszuler N. Measurement of the size and turnover of body glucose pool by isotope dilution method. Am J Physiol 187: 15-24, 1956[ISI].

27.   Van Nieuwenhoven, MA, Brouns F, and Brummer RM. The effect of physical exercise on parameters of gastrointestinal function. Neurogastroenterol Motil 11: 431-439, 1999[ISI][Medline].

28.   Williams, JH, Jr, Majer M, and Jacobson E. Relationship of mesenteric blood flow to intestinal absorption of carbohydrates. J Lab Clin Med 63: 853-863, 1964[ISI].


Am J Physiol Endocrinol Metab 281(4):E766-E771
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