Division of Endocrinology and Diabetology, Department of Medicine, Helsinki University Central Hospital, FIN-00029 HUCH, Helsinki, Finland
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ABSTRACT |
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Physical activity
increases the production of oxygen free radicals, which may consume
antioxidants and oxidize low-density lipoprotein (LDL). To determine
whether this occurs during strenuous aerobic exercise, we studied 11 well-trained runners who participated in the Helsinki City Marathon.
Blood samples were collected before, immediately after, and 4 days
after the race to determine its effect on circulating antioxidants and
LDL oxidizability in vitro. LDL oxidizability was increased as
determined from a reduction in the lag time for formation of conjugated
dienes both immediately after (180 ± 7 vs. 152 ± 4 min,
P < 0.001) and 4 days after (155 ± 7 min, P < 0.001)
the race. No significant changes in lipid-soluble antioxidants in LDL
or in the peak LDL particle size were observed after the race. Total
peroxyl radical trapping antioxidant capacity of plasma (TRAP) and uric
acid concentrations were increased after the race, but, except for
TRAP, these changes disappeared within 4 days. Plasma thiol
concentrations were reduced after the race. No significant changes were
observed in plasma ascorbic acid, -tocopherol,
-carotene, and
retinol concentrations after the marathon race. We conclude that
strenuous aerobic exercise increases the susceptibility of LDL to
oxidation in vitro for up to 4 days. Although the increase in the
concentration of plasma TRAP reflects an increase of plasma antioxidant
capacity, it seems insufficient to prevent the increased susceptibility
of LDL to oxidation in vitro, which was still observed 4 days after the race.
low-density lipoprotein oxidation; total peroxyl radical trapping antioxidant potential; lipids; low-density lipoprotein size
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INTRODUCTION |
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PHYSICAL ACTIVITY is associated with beneficial changes in circulating lipids and lipoproteins (10, 41), body weight, blood pressure, insulin sensitivity (22), and coagulation parameters (12, 13). These antiatherogenic changes could contribute to the reduced prevalence of cardiovascular disease in active individuals (4, 5, 16).
Heavy endurance exercise increases the rate of oxygen consumption in humans up to 20-fold, which induces oxidative stress and generates excess oxygen free radicals (6, 52). Under normal circumstances, the body has adequate antioxidant reserves to cope with increased production of free radicals. However, if the production of free radicals is excessive, as observed during strenuous aerobic exercise (28, 30), or if antioxidant defenses are severely hampered, the balance between prooxidants and antioxidants is lost. This may lead to tissue damage (20). Thus there is an apparent paradox between the benefits of heavy aerobic exercise on cardiovascular risk factors and the potentially deleterious consequences of free radicals generated during heavy exercise.
Oxidative modification of low-density lipoprotein (LDL) greatly increases its atherogenicity and is considered to be a key step in the development of atherosclerosis (53, 65). The susceptibility of LDL to oxidation in vitro has been reported to be associated with the severity of atherosclerosis (45). Also, autoantibodies against oxidized LDL seem to predict the progression of carotid atherosclerosis (47). Oxidative modification of LDL is induced by oxygen free radicals (39). Generally, LDL in the circulation is well protected against active oxidation by highly efficient plasma antioxidant defense mechanisms. Total peroxyl radical trapping antioxidant capacity of plasma (TRAP) reflects the total combined antioxidant capacity of all individual antioxidants. If, however, oxidative stress exceeds the capacity of the antioxidant defense, LDL may be oxidized.
Marathon running represents an extreme form of physical exercise and provides a model to study the effects of exercise-induced oxidative stress. In the present study, we determined the acute and postexercise effects of a marathon run on the susceptibility of LDL to oxidation in vitro, LDL particle size, antioxidants in LDL and plasma, and TRAP.
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SUBJECTS AND METHODS |
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Subjects. Eleven healthy male marathon
runners participating in the Helsinki City Marathon were studied.
Written informed consent was obtained after explanation of the purpose,
nature, and potential risks of this study to the subjects. The
experimental protocol was approved by the Ethical Committee of the
Minerva Foundation for Medical Research. Data of marathon runners were compared with those of an age-, sex-, and weight-matched healthy control group (n = 10) of untrained
subjects. Clinical characteristics of the study groups are summarized
in Table 1.
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Study design. The subjects ran a full marathon (42.2 km), except for one subject who interrupted the race after 81 min (16 km). The running times varied from 3.13 to 5.52 h. The mean energy consumption during the race was calculated by multiplying body weight in kilograms by the MET values (metabolic equivalent; work metabolic rate divided by resting metabolic rate; see Ref. 64) and duration of activity in hours (1). The subjects had no dietary restrictions during the days before the marathon. The runners were interviewed by a dietitian about their diet 24 h before the race and about their energy and lipid intake during and immediately after the race. The calculated average energy expenditure during the marathon was 3,579 ± 323 kcal. Total energy and nutrient intakes 24 h before the marathon and liquid nutrient intakes during the race were calculated using the Micro-Nutrica 2.0 PC program (The Social Insurance Institution, Turku, Finland; see Ref. 32). Twenty-four hours before the marathon, total energy intake averaged 3,074 ± 418 kcal, and comprised 66 ± 3% carbohydrates, 21 ± 2% fat, and 13 ± 1% protein. During the marathon, total energy intake averaged 249 ± 53 kcal, 98 ± 1% from carbohydrates, 1 ± 0.5% from fat, and 1 ± 0.5% from protein.
Venous blood samples were collected from every runner before,
immediately after, and 4 days after the race. Plasma was separated by
centrifugation and stored at 80°C until analyzed. To correct for possible changes in plasma volume that may occur during the marathon (41), the hematocrit was measured from fresh whole blood both
before and immediately after the race. The percentage change in plasma
volume was calculated based on the hematocrit measurements using the
formula of Beaumont (3). Immediately after the marathon, hematocrit was
significantly higher than before the race (43.9 ± 0.7% before vs.
45.1 ± 0.7% immediately after, P = 0.046). Consequently, the plasma volume decreased by 2.6 ± 1.1%
immediately after the race. The parameters measured in plasma immediately after the race were corrected for by taking into account this change in plasma volume.
Determination of LDL oxidation. LDL was isolated by short-run ultracentrifugation using a density gradient. Briefly, plasma (up to 5 ml) was adjusted with solid NaBr to a density of 1.5 g/ml and layered on the bottom of a centrifuge tube. This layer was then successively overlaid with 2.5 ml each of 1.21 and 1.063 g/ml NaCl solutions and 2.0 ml distilled water. All solutions contained 1 mg/ml of EDTA. The tubes were centrifuged in a Beckman SW 40 Ti rotor (Beckman Instruments, Fullerton, CA) in a Beckman L8-70 ultracentrifuge (Beckman, Palo Alto, CA) at 40,000 rpm at 4°C for 2.5 h. After centrifugation, the main lipoproteins were well separated from each other, and the distinct LDL band was carefully separated. Thereafter, EDTA was removed from LDL by using small dextran-sulfate affinity columns (Liposorber LA-15; Kaneka, Osaka, Japan), as previously described (60).
The oxidation of LDL in vitro was performed by using a modification of
the procedure described by Esterbauer et al. (15). The LDL oxidation
was initiated by adding freshly prepared
CuSO4 solution to a final
concentration of 10.64 µmol/l to the pure LDL solution. The kinetics
of LDL oxidation were determined by monitoring the change in absorbance
at 234 nm in a motorized six-cuvette cell-equipped Shimadzu
spectrophotometer (Shimadzu UV-1201; Shimadzu, Kyoto, Japan) connected
to a computer through a RS232 cable. Absorbance was recorded every 2 min. The change in absorbance at 234 nm over time could be divided into
three consecutive phases: lag phase, propagation phase, and
decomposition phase (15). The lag time (in min), the propagation rate
(in nmol · mg
LDL1 · min
1),
and the diene concentration (in nmol/mg LDL) were used as measures of
LDL to oxidation in vitro.
Measurement of plasma TRAP. TRAP was
determined spectrophotometrically using a recently validated method
(59). In this assay, we used 2',7'-dichlorofluorescein
diacetate (DCFH-DA) to follow the formation of free radicals during
decomposition of 2,2'-diazobis-(2-amidinopropane)dihydrochloride (AAPH). Free radicals were formed during thermal decomposition of AAPH
in water and followed by measuring the conversion of DCFH-DA to the
highly fluorescent dichlorofluorescein (DCF). The DCF
formation was measured at 504 nm in a Shimadzu spectrophotometer.
Plasma was mixed with PBS to a final dilution of 1%, followed by
addition of DCFH-DA to a final concentration of 14 µmol/l. The
reaction was started by adding AAPH to a final concentration of 56 mmol/l. The AAPH stock solution was stored at 20°C, thawed,
and kept in ice until added to the incubation. Trolox, 8.4 µmol/l, was used as an internal standard, and it was added during the
propagation phase when the absorbance had increased to 0.25-0.45.
DCF fluorescence or absorbance formation contains four phases. The
first lag phase is due to the antioxidants in the sample. After their
consumption by free radicals formed from AAPH, the reaction proceeds to
the first propagation phase. The second lag phase, which interrupts this propagation, is due to the addition of the internal standard, Trolox, to the incubation, and, in accordance, the second propagation of the reaction follows the consumption of the Trolox. The reaction gives the measured TRAP
(TRAPmea). A
theoretical calculated TRAP value
(TRAPcalc) can be estimated by
using the stoichiometric values for antioxidants after the measurement
of their concentrations in human samples. In the present study, the
TRAPcalc was calculated from four
main contributors of plasma antioxidant capacity, e.g.,
-tocopherol,
ascorbic acid, thiol, and uric acid. The difference between
TRAPmea and
TRAPcalc is due to antioxidants,
which are still unknown, or to the synergism among the various
antioxidants present in the plasma
(TRAPunknown).
Quantitation of LDL particle size.
Nondenaturing PAGE was performed on serum samples and was stored at
80°C using gels casted in our laboratory as previously
described in detail (34, 40). Gels were stained with Sudan Black B
lipid stain and were scanned with a computer-assisted laser scanning
densitometer (Personal Densitometer; Molecular Dynamics, Sunnyvale, CA)
using a 50-m pixel size and 12-bit signal resolution. Mean particle
diameter of the major LDL peak was determined by comparing the mobility of the sample with the mobility of a calibrated reference LDL standard
run on each gel. The particle diameters of the reference LDL
preparations were determined by electron microscopy. The coefficients of variation for intergel and intragel precisions for the control sample were 0.98 and 1.8%. The cutoff value for large and small LDL
particle diameter was set at 25.5 nm, and the size of the mean particle
diameter of the major LDL peak was also measured.
To avoid interassay variation, the susceptibility of LDL to in vitro oxidation, TRAP, and quantitation of LDL particle size was analyzed in the samples of each subject from different time points (before, immediately after, and 4 days after) at the same run.
Measurement of plasma antioxidants.
Plasma -tocopherol,
-carotene, and retinol were measured by
reverse-phase HPLC, as described by Schäfer-Elinder and Walldius
(50). We used a Hewlett-Packard reverse-phase HPLC column (ODS Hypersil
5 mm, 200 mm × 2.1 mm) connected to a Waters HPLC system. The
latter consisted of a M600 controller, M486 tunable ultraviolet
(UV)-absorbance detector, M717+ autosampler, and Millenium 2010 single-system chromatography manager (Waters, Milford, MA). The UV
detector was set at 326 nm for retinol, 292 nm for
-tocopherol, and
450 nm for
-carotene. Plasma ascorbic acid was measured using the
spectrophotometric method of Denson and Bowers (8). Plasma
protein-bound thiol (sulfhydryl groups) was determined as described by
Ellman (11). Plasma uric acid concentrations were measured by an
enzymatic colorimetric assay (Roche Unimate 5 UA; Roche).
Measurement of lipid-soluble antioxidants in
LDL. LDL was isolated by the same way as for the
determination of LDL oxidation. A volume containing ~100 µg LDL
protein was used to measure the concentrations of -tocopherol,
-carotene, and retinol in LDL. All further steps including HPLC
separation were the same as described above for plasma lipid-soluble
antioxidants. The
-tocopherol,
-carotene, and retinol
concentrations of LDL samples are expressed in relation to the total
LDL protein content (35) estimated concomitantly in each sample, i.e.,
nanomole antioxidant per milligram LDL protein.
Concentrations of lipid and lipoproteins. Plasma lipoproteins were isolated as previously described (23, 55) by using sequential ultracentrifugation (L8-70; Beckman) at the following densities: very-low-density lipoprotein <1.006 g/ml, intermediate-density lipoprotein 1.006-1.019 g/ml, LDL 1.019-1.063 g/ml, high-density lipoprotein (HDL) 1.063-1.210 g/ml.
The concentrations of cholesterol and triglycerides in plasma and lipoprotein subfractions were determined by enzymatic colorimetric assays (kit 07 3680 5 for triglycerides and 07 3664 3 for cholesterol; Hoffman-La Roche, Basel, Switzerland) in an autoanalyzer (Cobas Mira; Hoffmann-La Roche).
Maximal aerobic power and body
composition. Maximal aerobic power
(O2 max) was
determined by using a work-conducted upright exercise test with an
electrically braked cycle ergometer (Bosch ERG 220; Robert Bosch,
Berlin, Germany) combined with a continuous analysis of expiratory
gases and minute ventilation (EOS-Spint; Erich Jaeger, Wurtzburg,
Germany). Exercise was started at a workload of 50 W and was then
increased by 50 W at 3-min intervals until perceived exhaustion or
until a respiratory quotient of 1.10 was reached. The highest
O2 observed during a 30-s
period was defined as
O2 max.
Fat-free mass and the percent body fat were determined using bioelectrical impedance plethysmography (Bio-Electrical Impedance Analyzer System, model BIA-101A; RJL System, Detroit, MI).
Statistical analyses. Statistical analyses were done using the SYSTAT statistical package (SYSTAT, Evanston, IL). Values are given as means ± SE. The statistical analysis among the repeated observations measured before, immediately after, and 4 days after the race were tested by one-way repeated-measures ANOVA for multiple comparisons. When the overall model proved statistical significance (P < 0.05), the differences between the observations at different time points were tested by Bonferroni t-test. Statistical comparisons between the baseline values before the race in marathon runners and those of sedentary control subjects were performed using the unpaired Student's t-test. P values < 0.05 were considered as statistically significant. Data are shown as means ± SE.
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RESULTS |
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Susceptibility of LDL to oxidation in vitro and the
concentrations of lipid-soluble antioxidants in LDL.
The lag time for LDL oxidation was longer in the marathon runners
before the race than in the untrained subjects (180 ± 7 vs. 156 ± 10 min, P < 0.05). The lag
time, the propagation rate, and diene concentration before and after
the marathon run are shown in Table 2.
CuSO4-induced LDL oxidation in
vitro was accelerated at the early stages of the process, both
immediately and 4 days after the race (Fig. 1). As shown in Fig.
2A, the
lag time of conjugated diene formation was significantly reduced
immediately and 4 days after the race in each of the runners. The rate
of oxidation measured as the rate of propagation was also significantly
increased immediately after the race, but this change was abolished 4 days after the race. There were no significant differences in the
concentration of diene formation during LDL oxidation before or after
the race. The concentrations of the lipid-soluble antioxidants in LDL
are shown in Table 2. There were no significant changes of
-tocopherol,
-carotene, or retinol concentrations in LDL samples
after compared with before the marathon race. The mean size of the
major LDL peak was similar immediately and 4 days after the marathon
race compared with the baseline value before the race (Table 2).
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TRAP and circulating antioxidants.
TRAP was higher in the marathon runners before the race than in the
untrained subjects (1,072 ± 60 vs. 864 ± 80 µmol/l,
P < 0.05). Plasma TRAP and
antioxidant data in the runners are shown in Table
3. Immediately after the race,
both plasma TRAPmea and
TRAPcalc values corrected for
changes in plasma volume (Table 3 and Fig.
3) increased significantly compared with
baseline values before the race. The
TRAPmea increased in all subjects
(Fig. 2B), and the
TRAPcalc increased in all except two subjects (Fig. 2C). After 4 days, TRAPmea had decreased
significantly compared with the values immediately after the race, but
it was still significantly and 11.7% higher than the baseline value
before the race.
TRAPunknown was
increased significantly both immediately and 4 days after the race
(Fig. 3).
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Immediately after the race, plasma concentrations of uric acid were
higher than before the race (Table 3). Plasma thiol concentrations were
significantly lower immediately after than before the race (362 ± 15 vs. 315 ± 12 µmol/l, P < 0.001). Four days after the race, all of these parameters were
comparable to the baseline values before the race. We observed no
significant changes in plasma ascorbic acid, -tocopherol,
-carotene, and retinol concentrations immediately or 4 days after
the marathon race. The plasma ascorbic acid (63 ± 8 µmol/l),
-tocopherol (21.9 ± 1.7 µmol/l), thiols (331 ± 12 µmol/l), and uric acid (336 ± 12 µmol/l) in the untrained subjects were not significantly different from those in the runners.
The extent of training (km/wk) before the marathon correlated
positively with the plasma concentration of uric acid
(r = 0.848, P = 0.001) but inversely with the
plasma concentration of protein thiols before the marathon run
(r = 0.648,
P = 0.031).
Plasma lipids and lipoproteins. Plasma
lipids and lipoproteins were comparable in marathon runners before the
race and in untrained subjects, except for HDL cholesterol, which was
significantly higher in marathon runners before the race than in
untrained subjects (1.52 ± 0.10 vs. 1.25 ± 0.04 mmol/l,
P < 0.05). Plasma lipids and
lipoprotein concentrations in marathon runners before and after the run
are shown in Table 4. Immediately after the
race, the lipids and lipoproteins concentrations were not significantly different from the baseline value.
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DISCUSSION |
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In the present study, we found a clear reduction in the lag time of LDL oxidation in vitro when measured immediately and 4 days after a marathon race. The propagation rate of conjugated diene formation also increased significantly. These data indicate that strenuous exercise increases the susceptibility of LDL particle to oxidation in vitro, an effect that is potentially harmful. We also found an increase of plasma TRAP. The increase was explained by an increase in the concentration of the water-soluble antioxidant uric acid and the increase in the unknown component of TRAP, which is thought to reflect synergistic effects of antioxidants and the contribution of unidentified antioxidant mechanisms to TRAP (59, 63).
LDL oxidizability. Sanchez Quesada et al. (48) reported that intensive aerobic exercise increases LDL susceptibility to CuSO4-induced oxidation. Consistent with these data, in the present study, the marathon race increased the susceptibility of LDL particle to oxidation in vitro and decreased the lag time of conjugated diene formation. This change was not a transient phenomenon but persisted over 4 days. Consistent with previous cross-sectional data (49), the lag time of LDL to oxidation in marathon runners before the marathon run was longer than in sedentary controls. These data suggest that, while an acute single bout of strenuous exercise increased the susceptibility of LDL to oxidation, other training-associated factors counteract such changes. The degree of LDL oxidation reflects the net effects of its various prooxidants and endogenous antioxidants, the concentration of its oxidizable substrates, especially polyunsaturated fatty acids in LDL (44, 56), and LDL particle size (7). Any alterations of these factors will influence the oxidative modification of LDL and consequently may influence its atherogenicity.
Because oxidation of LDL does not occur until its natural endogenous
antioxidants have been consumed (14), the concentration of natural
endogenous antioxidants in LDL is an important determinant of the
susceptibility of LDL to oxidation. In the present study, the
concentrations of lipid-soluble antioxidants (-tocopherol,
-carotene, and retinol) in LDL were comparable before and after the
race. These data are consistent with previous cross-sectional data (49)
in which the content of lipid-soluble antioxidants in LDL did not
differ between trained and sedentary subjects. Vasankari et al. (61)
observed no changes in the LDL TRAP during a 31-km run or during a
marathon run. Because the concentration of lipid-soluble antioxidants
in LDL is the major determinant of the LDL TRAP, these data suggest
that acute exercise does not influence the antioxidant defense capacity
of LDL particles. Small dense LDL particles are more prone to oxidation
than large buoyant particles and are an independent risk predictor of
cardiovascular disease (7). In the present study, LDL particle peak
size was unaffected by the marathon race. This finding agrees with
previous studies in which acute exercise did not significantly change
the concentrations of LDL subfractions (2, 48).
Taken together, we cannot explain the observed increase in the
susceptibility of LDL to oxidation in vitro by changes in either the
concentrations of lipid-soluble antioxidants in LDL or LDL particle
size. Of the lipid-soluble antioxidants in LDL, vitamin E seems to be a
more effective antioxidant than -carotene (18, 25, 43). Ubiquinol-10
may, however, protect human LDL even more efficiently against lipid
peroxidation than vitamin E (54). Ubiquinol-10 in LDL or plasma was not
determined in the present study. Recent studies have reported that HDL
can protect LDL from oxidation both in vitro and in vivo (31, 36). Some
studies suggest that paraoxonase, an HDL-associated enzyme, may be
responsible for the antioxidative action of HDL (37). However,
mechanisms via which HDL can protect LDL from oxidation and its
contribution to TRAP are still poorly understood. HDL particles seem to
be important for transport of circulating plasma lipid hydroperoxides. Mackness et al. (36) reported that inhibition of the formation of LDL
lipid peroxides is dependent on the concentration of HDL. It was shown
that the increased resistance to oxidation of LDL in trained subjects
(compared with sedentary controls) may be due to their increased plasma
HDL cholesterol concentration (49). However, in the present study, the
plasma HDL concentrations were similar before and immediately after the
marathon run but whether the antioxidant properties of HDL were altered
cannot be determined after a marathon run.
Plasma TRAP and circulating antioxidants. In the present study, plasma TRAPmea was significantly increased both immediately and 4 days after the race. In addition, plasma TRAP was higher in marathon runners before the race than in untrained controls. Vasankari et al. (61) have also reported that acute exercise significantly increases serum TRAP. These data suggest that acute exercise activates antioxidant defenses in the body. This activation can be viewed as an adaptive defensive mechanism to cope with increased oxidative stress. Regarding the causes of the increase in TRAP, we found an increase in the concentration of uric acid (Table 3) and in the unidentified antioxidant capacity in plasma (TRAPunknown; Fig. 3).
During exercise, energy-rich phosphates are utilized, resulting in hypoxanthine accumulation in tissues (52). Conversion of hypoxanthine to uric acid does not occur until an adequate oxygen supply has been reinstituted. The latter process is associated with the formation of toxic oxygen free radicals (52). On the other hand, uric acid possesses antioxidant activity (63) and serves as a free radical scavenger in vivo (24). Plasma uric acid can trap peroxyl radicals in the aqueous phase and contribute to plasma antioxidant defenses (63). Some previous studies have suggested that acute exercise increases plasma uric acid concentrations (42, 46). This was confirmed in our study in which plasma uric acid increased during exercise, but the change was only temporary and was not seen 4 days after the race. However, we observed a highly significant correlation between the extent of training and the baseline concentration of plasma uric acid before the race. These data suggest that exercise increases uric acid concentration. This increase could reflect enhanced purine oxidation in muscle (46).
Thiols (sulfhydryl groups) are known to scavenge aqueous peroxyl radicals (17, 63). In the present study, the plasma thiol levels were significantly reduced immediately after the marathon run in all subjects (Fig. 2D). These findings are similar to the data reported by Inayama et al. (26), who found acute exercise to decrease plasma thiol concentrations. Wayner et al. (63) reported that plasma thiols are the first antioxidants consumed during 2,2'-azobis-(2-amidopropane)hydrochloride-initiated peroxidation of plasma. Thus the observed decrease of the plasma thiol concentrations could reflect their oxidation by exercise-induced free radicals in the aqueous phase of plasma. Physical training may also chronically deplete plasma thiols, since we found an inverse correlation between the extent of training and baseline plasma thiol concentrations.
The effect of acute exercise on plasma ascorbic acid concentrations has
been variable. Both increases (46, 62) and no change (19) in plasma
ascorbic acid concentrations have been reported in different studies.
In the present study, the plasma ascorbic acid concentration tended to
increase after the race, but the change was not significant. Some (46,
61), but not all (38, 62) previous studies, have suggested that
exercise increases plasma -tocopherol concentrations. The increase
of plasma
-tocopherol induced by exercise may be due to a shift in
the interorgan distribution where liver and adipose tissue may be
donors and muscles and heart are vitamin E receivers (58). In our
study, immediately after the race, the plasma
-tocopherol concentration, corrected for the plasma volume shifts, was not significantly increased. Several other circulating plasma antioxidants and antioxidant enzymes (
-carotene, retinol, ubiquinol-10,
bilirubin, glutathione, glutathione peroxidase, catalase, superoxide
dismutase, transferrin, and ceruloplasmin) also contribute to the
plasma antioxidant capacity (21, 27-30, 46, 51, 62, 63). The relative contribution of these components to TRAP is, however, trivial
(63). In the present study, exercise had no effect on the plasma
-carotene and retinol concentrations. These findings are consistent
with previous studies in which acute exercise did not change plasma
-carotene (46) and retinol levels (46, 61).
Plasma antioxidants can also act synergistically in vivo to provide more protection against free radical damage than could be provided by any single antioxidant alone (63). In the present study, before the race, TRAPcalc and TRAPunknown amounted to 68 and 32% of the TRAPmea (Fig. 3). In contrast, after the marathon run, the increase of TRAPmea was due to a 73% increase of TRAPunknown, whereas the increases in the concentrations of uric acid and other antioxidants accounted for 27%. This marked increase of TRAPunknown suggests that acute exercise may activate unidentified antioxidant defenses as a physiological adaptive response against oxidative stress. Even so, neither of the components of TRAP seem sufficient to prevent the increased susceptibility of isolated LDL particle to oxidation in vitro.
Lipid and lipoproteins. Previous data (10) have shown that regular physical training is associated with lowering of plasma total triglycerides, total cholesterol and LDL concentrations, and an increase in the plasma HDL cholesterol concentration. The effect of a single bout of exercise on lipid concentrations has, however, been highly variable (10, 41). For example, increases (9), decreases (57), or no change (33) in plasma total triglycerides and/or total cholesterol have been reported in different studies. The changes of plasma LDL cholesterol concentrations have also been variable, whereas the increase in HDL cholesterol has been more consistent (9, 33, 57). In the present study, the concentrations of total cholesterol, triglycerides, and LDL cholesterol remained unchanged immediately and 4 days after the marathon run. The plasma HDL cholesterol concentration immediately after the race was slightly, but not significantly, increased compared with the baseline value.
Conclusion. A single bout of heavy endurance physical activity increases TRAP and some (uric acid, TRAPunknown) of its components. These increases can be viewed as an adaptive defensive mechanism against exercise-induced oxidative stress. The decrease of the plasma thiol concentration immediately after the race may reflect its role as a first-line defense mechanism and consumption by reactive oxygen species in the aqueous phase of plasma. The marathon run also increased the susceptibility of LDL to oxidation in vitro, and this change persisted over 4 days. This increased susceptibility of LDL to oxidation in vitro was not explained by changes in either endogenous lipid-soluble antioxidants in LDL or LDL particle size.
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ACKNOWLEDGEMENTS |
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We are grateful to Helinä Perttunen-Nio, Sari Hämäläinen, Kati Tuomola, Ulla Minkkinen, Ritva Marjanen, Kikka Runeberg, and Leena Lehikoinen for skillful technical assistance. We are grateful to Dr. Mikko Syvänne and Juha Vakkilainen for helpful advice on the statistical analyses. We also thank Maaria Puupponen for help on this manuscript.
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FOOTNOTES |
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This work was supported by grants from the Helsinki University Central Hospital EVO Funds, the Academy of Finland (H. Yki-Järvinen, M.-R. Taskinen), Finska Läkaresällskapet (R. Bergholm), and the Sigrid Juselius Foundation (H. Yki-Järvinen, M.-R. Taskinen).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. §1734 solely to indicate this fact.
Address for correspondence: M.-R. Taskinen, Div. of Endocrinology and Diabetology, Dept. of Medicine, Haartmaninkatu 4, PO Box 340, FIN-00029 HUCH, Helsinki, Finland (E-mail: mataskin{at}helsinki.fi).
Received 14 July 1998; accepted in final form 19 February 1999.
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