The following is the abstract of the article discussed in the subsequent letter.
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ABSTRACT |
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Adey, Deborah, Rajiv Kumar, James T. McCarthy, and K. Sreekumaran
Nair. Reduced synthesis of muscle proteins in chronic renal failure.
Am. J. Physiol. Endocrinol. Metab. 278: E219-E225, 2000.Muscle wasting and weakness occur frequently in patients with
chronic renal failure. The mechanism(s) by which these abnormalities occur is unclear. We hypothesized that such findings were due to
defective muscle protein synthesis. We measured synthetic rates of
mixed muscle proteins, myosin heavy chain, and mitochondrial proteins
in serial muscle biopsy samples during a continuous infusion of
L[1-13C]leucine from 12 patients with chronic
renal failure and 10 healthy control subjects under identical study
conditions. Patients with chronic renal failure have significantly
lower synthetic rates of mixed muscle proteins and myosin heavy chain
(27 and 37% reductions, respectively, P < 0.05 and
P < 0.02). Significant declines in the synthetic rates
of muscle mitochondrial protein (27%) (P < 0.05),
muscle cytochrome c-oxidase activity (42%) (P < 0.007), and citrate synthase (27%) (P < 0.007)
were also observed in patients with chronic renal failure. The
synthetic rates of muscle proteins and activity of mitochondrial
enzymes were negatively correlated to the severity of renal failure.
These results indicate that in chronic renal failure there is a
decrease in the synthesis of muscle contractile and mitochondrial
proteins and a decrease in muscle mitochondrial oxidative enzymes.
Reduced synthetic rate of several muscle proteins is the likely
biochemical basis of muscle loss and muscle weakness in people with
chronic renal failure.
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LETTER |
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Why Is Muscle Protein Synthesis, But Not Whole Body Protein Synthesis, Reduced in CRF Patients?
To the Editor: Adey et al. (1) recently published an interesting study, unique in its comparison of systemic and local protein metabolism in chronic renal failure (CRF). Using muscle biopsies, they found a 27% reduced mixed muscle protein synthesis and a 37% reduction of myosin heavy-chain synthesis. The systemic findings obtained with whole body leucine kinetics show overall protein metabolism to be similar to that of normal controls. This appears at first sight to be a discrepancy and receives little attention in the discussion. We believe, however, that it may be a crucial finding. Possibly the decreased muscle protein synthesis is not a defect but an adaptive mechanism. Thus, by decreasing protein synthesis in muscle, amino acids are made available for synthesis of proteins serving other purposes, such as immunological ones. If uremic toxins would aspecifically cause a decrease in muscle protein synthesis, they would also be likely to cause decreased systemic protein synthesis. Because this is not the case, the effect may be similar to the one described by Mansoor et al. (2), who found a 50% reduction of muscle protein synthesis whereas whole body protein synthesis increased by 28%. In these experiments, there was an evident shift of protein synthesis from muscle protein to the immune system. This is probably also the case in a more chronic manner for CRF patients (3). The possible connections between low-grade inflammation of uremia on the one hand and the uremic complications on the other hand are beginning to attract more attention. It may well be that low-grade inflammation is a major cause not only of the accelerated atherosclerosis of uremia but also of the changes in protein metabolism described by Adey et al. ![]() |
REFERENCES |
---|
1.
Adey, D,
Kumar R,
McCarthy JT,
and
Nair KS.
Reduced synthesis of muscle proteins in chronic renal failure.
Am J Physiol Endocrinol Metab
278:
E219-E225,
2000
2.
Mansoor, O,
Cayol M,
Gachon P,
Boirie Y,
Schoeffler P,
Obled C,
and
Beaufrère B.
Albumin and fibrinogen syntheses increase while muscle protein synthesis decreases in head-injured patients.
Am J Physiol Endocrinol Metab
273:
E898-E902,
1997
3.
Stenvinkel, P,
Heimburger O,
Lindholm B,
Kaysen GA,
and
Bergstrom J.
Are there two types of malnutrition in chronic renal failure? Evidence for relationships between malnutrition, inflammation and atherosclerosis (MIA syndrome).
Nephrol Dial Transplant
15:
953-960,
2000
Jorden M. Veeneman, | ||||||||||||
Paul E. de Jong, | ||||||||||||
Roel M. Huisman, Division Nephrology, Department of Internal Medicine, University Hospital Groningen 9713 GZ Groningen, The Netherlands | ||||||||||||
Dirk-Jan Reijngoud, Department of Pediatrics, Faculty of Medicine, University of Groningen 9700 RB Groningen, The Netherlands |
To the Editor: In their Letter to the Editor,
Veeneman et al. proposed an interesting hypothesis about our
observation (1) that, whereas fractional synthetic rates
of muscle proteins (myosin heavy, mitochondrial protein, and mixed
muscle proteins) are lower in people with chronic renal failure (CRF),
no differences in whole body leucine kinetics between CRF and healthy
control subjects were observed. In the absence of any direct
measurements of synthesis rate of nonmuscle proteins, the above
hypothesis remains to be tested. The comparison of whole body data
between two groups of subjects with different body compositions must be
interpreted with caution. Unlike fractional synthesis rates of muscle
proteins, whole body leucine kinetics are normalized for fat-free mass
(FFM). FFM was measured by dual-energy X-ray absorptiometry (DEXA).
DEXA measurement of FFM does not distinguish between muscle mass and water. It is likely that we may have overestimated FFM on CRF patients
who may have water retention. A similar situation exists in older
subjects with sarcopenia, in whom FFM measurement by DEXA overestimated
lean mass because of increased body water (2). It is also
unclear whether changes in the composition of nonmuscle tissues occur
in people with CRF. It is, therefore, prudent not to make any
conclusions on whole body leucine kinetic values on the basis of
cross-sectional comparison between populations with different body
compositions. The proposed hypothesis by Veeneman et al. could be
tested by directly measuring synthetic rates of proteins involved in
the immunological or inflammatory responses in people with CRF.
REPLY
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REFERENCES |
---|
1.
Adey, D,
Kumar R,
McCarthy JT,
and
Nair KS.
Reduced synthesis of muscle proteins in chronic renal failure.
Am J Physiol Endocrinol Metab
278:
E219-E225,
2000
2.
Proctor, DN,
O'Brien PC,
Atkinson EJ,
and
Nair KS.
Comparison of techniques to estimate total body skeletal muscle mass in people of different age groups.
Am J Physiol Endocrinol Metab
277:
E489-E495,
1999
K. Sreekumaran Nair, Division of Endocrinology Mayo Clinic and Foundation Rochester, Minnesota 55905 |
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