Reduced insulin-stimulated glucose transport in denervated muscle is associated with impaired Akt-alpha activation

Jason J. Wilkes and Arend Bonen

Department of Kinesiology, University of Waterloo, Waterloo, Ontario, N2L 3G1 Canada


    ABSTRACT
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Insulin signaling was examined in muscle made insulin resistant by short-term (24-h) denervation. Insulin-stimulated glucose transport in vitro was reduced by 28% (P < 0.05) in denervated muscle (DEN). In control muscle (SHAM), insulin increased levels of surface-detectable GLUT-4 (i.e., translocated GLUT-4) 1.8-fold (P < 0.05), whereas DEN surface GLUT-4 was not increased by insulin (P > 0.05). Insulin treatment in vivo induced a rapid appearance of phospho[Ser473]Akt-alpha in SHAM 3 min after insulin injection. In DEN, phospho[Ser473]Akt-alpha also appeared at 3 min, but Ser473-phosphorylated Akt-alpha was 36% lower than in SHAM (P < 0.05). In addition, total Akt-alpha protein in DEN was 37% lower than in SHAM (P < 0.05). Akt-alpha kinase activity was lower in DEN at two insulin levels tested: 0.1 U insulin/rat (-22%, P < 0.05) and 1 U insulin/rat (-26%, P < 0.01). These data indicate that short-term (24-h) denervation, which lowers insulin-stimulated glucose transport, is associated with decreased Akt-alpha activation and impaired insulin-stimulated GLUT-4 appearance at the muscle surface.

GLUT-4; soleus; tibialis anterior


    INTRODUCTION
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

SKELETAL MUSCLE IS A MAJOR SITE for insulin-dependent disposal of circulating glucose. In this tissue, insulin signaling to stimulate glucose transport occurs through a network of protein-protein interactions (26, 49) that promote GLUT-4 glucose transporter translocation from intracellular sites to T-tubules and plasma membranes (18). Insulin receptor substrate (IRS) proteins (IRS-1/IRS-2) couple insulin receptor (IR) activity to metabolic events controlled by phosphatidylinositol-3' kinase (PI 3-kinase) (2, 3). PI 3-kinase activation is a key signal that promotes GLUT-4 translocation (10, 40, 44). However, insulin-stimulated PI 3-kinase activation alone is insufficient to stimulate glucose transport (27), implying that additional signaling protein(s) beyond PI 3-kinase are involved in stimulating glucose transport.

One target of PI 3-kinase is a plecktrin homology domain containing serine/threonine kinase Akt [protein kinase B, or related to A and C (rac) protein kinase]. Akt lies downstream of PI 3-kinase in the insulin-signaling pathway (9, 28) and becomes active when phosphatidylinositol-dependent kinases PDK1 and PDK2 phosphorylate Akt on specific kinase activation sites (14). Overexpression of constitutively active Akt protein increases GLUT-4 translocation independently of insulin stimulation in 3T3-L1 adipocytes (31) and rat adipocytes (15). Insulin-induced Akt-alpha activation is thought to promote an increase in surface glucose transporters in insulin-responsive cells and tissues (21, 31, 45). In the skeletal muscle from patients with non-insulin-dependent diabetes mellitus, Akt-alpha kinase activity is reported to be reduced in concert with a reduction in insulin-stimulated glucose transport (33). Furthermore, in insulin-resistant skeletal muscle of hyperglycemic Goto-Kakizaki rats, insulin-stimulated Akt-alpha kinase activity is also reduced (32, 42). However, when the blood glucose concentrations in Goto-Kakizaki rats were normalized with phlorizin treatment, Akt-alpha kinase activity was restored, and insulin-stimulated glucose transport was improved (32, 42). Thus Akt-alpha may be a central component of insulin resistance that can develop in skeletal muscle.

We (23, 38, 39) and others (12, 19, 25, 46) have shown that, when muscle activity is completely eliminated by severing the motor nerve (denervation), insulin-stimulated glucose transport is markedly reduced. The insulin resistance observed in denervated muscle develops rapidly, because insulin-stimulated glucose transport rates are decreased within a few hours after the muscles have been denervated (~6 h) (48). After 3 days of denervation, the large reductions in the total GLUT-4 content (<= 80%) (23, 37, 38) and reduced insulin-stimulated PI 3-kinase activity (19) can account for a considerable fraction of the reduced insulin-stimulated glucose transport. However, the reduction in insulin-stimulated glucose transport observed after 24 h of denervation (19, 25) occurs before there is a decrease in the total amount of GLUT-4 protein (4, 12, 25) or PI 3-kinase activation (11, 19). To date, no mechanism has been identified to account for insulin resistance in 24-h-denervated muscles.

Because Akt-alpha has been implicated as a key insulin signaling protein for regulating GLUT-4 translocation, we examined whether the loss of insulin-stimulated glucose transport in short-term (24-h)-denervated muscle, in which GLUT-4 content is not altered, is associated with 1) impaired insulin-stimulated Akt-alpha activation and 2) reduced GLUT-4 translocation.


    MATERIALS AND METHODS
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Materials

Polyclonal sheep anti-rat Akt-alpha antibody, anti-phospho [Ser473]Akt-alpha antibody, protein kinase A inhibitor peptide, and crosstide Akt-specific substrate were obtained from Upstate Biotechnology (Lake Placid, NY). A polyclonal immuno-A purified GLUT-4 antibody against a region in the COOH terminus was obtained from East Acres Biologicals (Southbridge, MA). Horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG was obtained from Amersham Life Science (Buckingham, UK). HRP-conjugated rabbit anti-sheep was obtained from Rockland (Gilbertsville, PA). Protein G Sepharose-4 fast flow was obtained from Amersham Pharmacia Biotechnology (Uppsala, Sweden). Protein A agarose was obtained from BIO/CAN Scientific (Mississauga, ON, Canada). [gamma -32P]ATP was obtained from NEN Life Science (Boston, MA). 2-Deoxy-D-[1,2-3H]glucose was obtained from American Radiolabeled Chemicals (St. Louis, MO). [14C]mannitol and bacitracin were obtained from ICN (Costa Mesa, CA). Microcystin and LY-294002 were obtained from Calbiochem (San Diego, CA). Leupeptin, aprotinin, phenylmethylsulfonyl fluoride (PMSF), chymotrypsin-free trypsin, soybean trypsin inhibitor (SBTI), and pepstatin A were obtained from Boehringer Mannheim (Laval, Quebec, Canada). Porcine insulin (Iletin II) was obtained from Eli Lilly. RIA grade bovine serum albumin (BSA), potassium cyanide (KCN), pyruvate, and other chemicals were obtained from Sigma (St. Louis, MO).

Animals

Male Sprague-Dawley rats weighing 200-230 g were housed in a temperature-controlled environment and were maintained on a 12:12-h reverse light-dark cycle. The animals were provided with a rat chow diet ad libitum and tap water. All experimental procedures were approved by the Committee on Animal Care at the University of Waterloo.

Hindlimb muscle denervations. The hindlimb muscles in one limb were denervated as we have previously described (38). Briefly, rats were anesthetized under halothane gas after a subcutaneous injection of buprenorphine (0.03 mg/kg) analgesic. A small superficial incision was made on one leg, the sciatic nerve was located, and ~3 mm of the nerve were removed. A sham operation was performed on the contralateral leg. Incisions in both legs were closed with surgical clips, and a topical disinfectant was applied to the skin. After rats had recovered (<= 1 h), food and water were provided. Experiments were performed 24 h later on overnight-fasted rats.

Animal preparation. Rats were anesthetized with 65 mg/kg pentobarbital sodium and placed on a heating pad at a low temperature setting, and hindlimb muscles were exposed by carefully cutting free the exterior skin. Soleus muscles were removed for determination of glucose transport and surface GLUT-4 measurements. Approximately 15 min later, the descending aorta in rats was injected with 0.1 ml saline (+0.1% BSA) plus insulin or the equivalent volume of saline (+0.1% BSA) alone, followed by a rapid dissection of the tibialis anterior (TA) muscle at two specific time points (3 and 5 min) in different experiments. After the denervated and sham TA muscles were dissected from hindlimbs, the red TA (RTA) portion was quickly separated from the white TA compartment, immersed in liquid N2, and kept at -80°C for later use.

Insulin-Stimulated Glucose Transport in Incubated Soleus Strips

Soleus muscles were cut into thin lengthwise strips suitable for in vitro incubations (5). Strips were preincubated for 1 h at 29°C in Krebs-Henseleit buffer (KHB) with 32 mM mannitol, 8 mM D-glucose, and 0.1% BSA. Porcine insulin was added to the incubation medium at a maximal stimulating concentration of 400 µU/ml for 30 min. Before glucose transport measurements, D-glucose was removed by washing strips twice for 5 min each in a glucose-free KHB with 38 mM mannitol and 2 mM pyruvate. The uptake of 2-deoxyglucose was determined with 2 mM pyruvate (1.5 µCi), 2-deoxy-D-[3H]glucose (1 mM and 0.1 µCi), and [14C]mannitiol (37 mM) for 10 min. Strips were removed rapidly, rinsed in 0.9% ice-cold saline, cut free of tendons, and snap frozen in liquid N2. Muscles were stored at -80°C until analyzed for 14C and 3H in digested muscle extract (6, 7).

GLUT-4 AND AKT-alpha Determination by Western Blotting

Total Akt-alpha and total GLUT-4 protein. For GLUT-4 determinations, soleus muscle was prepared as described elsewhere (22, 29). For Akt-alpha determinations, RTA muscles (50-80 mg) were homogenized in ice-cold homogenization buffer (50 mM Tris, pH 7.5, 110 mM sodium tetrapyrophosphate, 11 mM EDTA, 110 mM sodium fluoride, 10 mM sodium orthovanadate, 1% Triton X-100, 200 mM PMSF, 10 mg/ml aprotinin, 1 mg/ml leupeptin, and 1 mg/ml pepstatin A). Western blotting was used to determine GLUT-4 and Akt-alpha content. Briefly, samples were separated by SDS-PAGE on a 12% gel and transferred to an Immobilon membrane (Millipore) by electromembrane transfer for 90 min. Membranes were blocked overnight in 5% nonfat dry milk (NFDM) made in TBS (pH 7.6). Proteins were detected by incubation of blocked membranes with an anti-GLUT-4 polyclonal immuno-A purified antibody (1:7,000) or anti-rat Akt-alpha antibody (1:1,000) followed by HRP-conjugated anti-rabbit IgG diluted (1:2,000) in TBS, pH 7.6, for GLUT-4 detection or with HRP-labeled anti-sheep IgG diluted (1:2,000) in 5% NFDM for Akt-alpha detection. GLUT-4 and Akt-alpha were visualized with an enhanced chemiluminescence system (Amersham Life Science) according to the manufacturer's instructions. Western blots were quantified by a Macintosh LC with an Abaton scanner and appropriate software (Scan Analysis, Biosoft, Cambridge, UK).

Surface detectable GLUT-4. The method for detection of surface GLUT-4 in skeletal muscle was adapted from a procedure used with rat adipocytes (16, 51). This method is based on the principle that exogenous trypsin cuts surface-accessible GLUT-4 at a predicted trypsin cleavage site in the exofacial loop of the GLUT-4 transporter. Trypsin-cleaved GLUT-4 appears on a blot at a lower molecular weight than native GLUT-4 protein when the fragmented GLUT-4 is detected with antibodies to the GLUT-4 carboxy terminus (16). GLUT-4 fragments migrate to 35 kDa instead of the 46-kDa position of nontrypsinized native GLUT-4. We have previously used this procedure to detect surface GLUT-4 in adipocytes (51).

To apply the method to skeletal muscle, soleus muscles were cut into strips and preincubated in vials with KHB for 45 min at 35°C. Insulin (400 µU/ml) was added to one-half of the vials for 10 min. All vials received 2 mM KCN for another 20 min to stop surface membrane proteins from recycling with internal protein pools. Strips were treated with 1 mg/ml trypsin and continued to incubate for an additional 30 min. Strips were washed (2 × 5 min) in a buffer free of exogenous trypsin with 1 mg/ml SBTI added; they were then quickly frozen in liquid N2 and stored at -80°C for later use. Strips were prepared with the standard homogenization protocol described above for GLUT-4 detection by Western blotting, except that the homogenization buffer also contained 1 µg/ml SBTI. Blots were quantified by means of a scanner connected to a Macintosh computer with appropriate software.

Akt-alpha [Ser473] phosphorylation with in vivo insulin treatment. Saline- and insulin-injected rats were used to determine basal (non-insulin-stimulated) and activated Akt-alpha [Ser473] phosphorylation levels, respectively. Muscles were homogenized in ice-cold buffer 1 [150 mM NaCl, 50 mM TRIS, pH 7.5, 30 mM sodium pyrophosphate, 10 mM sodium fluoride, 1 mM dithiothreitol (DTT), 10% (vol/vol) glycerol, 1% Triton X-100, 1 mg/ml bacitracin, 200 mM PMSF, 10 mg/ml aprotinin, 1 mg/ml leupeptin, 1 mg/ml pepstatin A, 1 µM microcystin]. Homogenates were spun at 15,000 rpm (70 Ti, Sorvall) for 1 h at 4°C. Samples were resolved on a 6% gel by SDS-PAGE and transferred to an Immobilon membrane (Millipore) by electromembrane transfer for 90 min. Membranes were blocked in 5% nonfat dry milk (1 h) and probed with anti-phospho[Ser473]Akt-alpha antibody (1 h) followed by HRP-labeled anti-sheep IgG secondary antibody (1 h). Phospho[Ser473]Akt-alpha was visualized and quantified as described above.

Measurements of Akt-alpha Kinase Activity in Vivo

Akt-alpha kinase activity was determined as described by Krook et al. (32). For these experiments, rats were injected with 0.1 or 1 U insulin to generate physiological or supraphysiological plasma insulin concentrations, respectively. RTA muscles were dissected 5 min after injection, at a time when insulin injections are known to maximally activate muscle Akt-alpha (48). Thus the dissection was completed rapidly, and muscles were frozen quickly in liquid N2. To determine plasma insulin levels from injected rats, three blood samples of ~0.1 ml each were withdrawn. Muscles were homogenized as described above for Akt-alpha [Ser473] phosphorylation measurements. Anti-Akt-alpha antibody was agitated with protein G Sepharose beads in buffer 1 for 1 h to form an anti-Akt-alpha -protein G immune complex. Aliquots of muscle homogenate were rotated with protein G Sepharose beads (50 µl/ml) and pulsed in a bench-top Sorvall (12,000 g) to reduce nonspecific binding. The activated Akt kinase was immunoprecipitated from precleared sample (500 µg) by rotation with the anti-Akt-protein G immune complex overnight at 4°C. Akt-alpha protein immunoprecipitates were collected by centrifugation, washed 4 times in buffer 2 (1 M NaCl, 25 mM HEPES, pH 7.6, 1 mM DTT, 0.1% BSA, 10% glycerol, 1% Triton X-100) to remove unbound protein, and washed 2 times in kinase buffer 3 (50 mM Tris, pH 7.5, 10 mM MgCl2, 1 mM DTT). The final buffer was carefully removed, and beads were completely dried and resuspended in 30 µl kinase buffer 3 supplemented with 100 µM cold ATP, 2 µCi [gamma -32P]ATP, 17 nM protein kinase A inhibitor, and 100 µM Akt-specific crosstide peptide (GRPRTSSFAEG). After 30 min of gentle shaking at 30°C, reactions were terminated by placing tubes into ice. 32P incorporation into crosstide peptide was determined by resolving 25 µl of reaction mixture on a 40% urea-based acrylamide gel. Autoradiographs were generated by exposing the dry gel to scientific imaging film X-OMAT AR (Kodak), and the band corresponding to the peptide substrate was quantified by densitometry.

Protein Assay

All protein concentrations were determined in triplicate by bicinchoninic acid assay (Sigma), with BSA as a standard.

Plasma Insulin

Insulin levels were determined with the use of an insulin radioimmunoassay kit (DPC, Los Angles, CA) according to the manufacturer's directions.

Statistical Analysis

Data were analyzed by use of analysis of variance and t-test, as appropriate. All data are reported as means ± SE.


    RESULTS
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

Glucose Transport in Incubated Soleus Strips

Glucose transport was determined in soleus muscles in vitro. In addition, incubated control and denervated soleus muscles were also used to compare total GLUT-4 and the surface GLUT-4 in basal and insulin-stimulated muscles.

In the absence of insulin (basal), 2-deoxyglucose transport was not different between 24-h-denervated and control soleus muscle (P > 0.05) (Fig. 1). Insulin treatment increased soleus muscle glucose transport 2.2-fold in control (P < 0.01) but only 1.7-fold in denervated soleus muscles (28% reduction, P < 0.01; Fig. 1). In a separate experiment, when insulin-stimulated glucose transport was determined by hindlimb perfusion, the denervated RTA muscle showed a similar reduction (-27%) in glucose transport (4.0 ± 0.28 vs. 2.92 ± 0.21 µmol · g-1 · 10 min-1, P < 0.05).


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Fig. 1.   Insulin-stimulated 2-deoxyglucose transport in vitro in soleus muscle from 24-h-denervated rats. Values are means ± SE; n = 8-12 soleus strips for basal and insulin-treated muscle from denervated and control legs. Insulin-treated groups are significantly different from their respective basal, * P < 0.01; dagger  denervated group (hatched bars) treated with insulin alone vs. same treatment in control group (solid bars), P < 0.05.

Insulin Signaling through AKT-alpha

Insulin was injected in vivo to activate muscle Akt-alpha . Basal Akt-alpha kinase activity (Fig. 2) was not significantly different from nonspecific activity [i.e., nonspecific = enzyme activity measured in protein G beads that were not conjugated with the Akt-alpha antibody (data not shown)]. Basal Akt-alpha kinase activity was set at 100% for both groups.


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Fig. 2.   Insulin activation of Akt-alpha kinase in vivo in red tibialis anterior (RTA) muscle from 24-h-denervated rats. Saline treatment is set = 1 for both groups (n = 3). Significant differences are denoted *P < 0.05 and ** P < 0.01 vs. saline. For insulin-treated animals, data from insulin treatments are paired (n = 6). Means in control group are different from those in denervated group, dagger  P < 0.05, Dagger  P < 0.01.

Insulin-stimulated Akt-alpha kinase activity. Plasma insulin concentrations were elevated to high physiological levels or to supraphysiological levels after insulin injections (Table 1). In control muscle, 0.1-U insulin injections increased Akt-alpha kinase activity (Fig. 2) 2.3-fold (P < 0.05), whereas 1-U insulin injections increased Akt-alpha kinase activity 3.4-fold (P < 0.01). In denervated muscle, Akt-alpha kinase activity was found to be 22 (P < 0.05) and 26% lower (P < 0.01) after 0.1-U and 1-U insulin injections, respectively.

                              
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Table 1.   Plasma insulin before and 7 minutes after exogenous insulin administration by intravascular injection

Akt-alpha [Ser473] phosphorylation and total immunoreactive Akt-alpha protein. Insulin-stimulated Akt-alpha phosphorylation occurred in a time-dependent manner (Fig. 3). Phospho[Ser473]Akt-alpha protein level was significantly higher in control muscle at 3 min (P < 0.05) than in denervated muscle. In addition, the total Akt-alpha protein (Fig. 4) was decreased (-37%) in denervated muscle (P < 0.05).


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Fig. 3.   Effect of insulin injections on Akt-alpha [Ser473] phosphorylation in RTA muscle from 24-h-denervated rats. Blot shows Akt-alpha [Ser473] bands from one representative experiment. p-Akt, phospho[Ser473]Akt-alpha . The Akt-alpha [Ser473] band is not detectable in saline-injected animals. Basal arbitrary scanning units represent background. Values are represented as means ± SE of arbitrary scanning units; n = 6 RTA muscles at 3 min; n = 3 RTA muscles at 5 min. Control (diamonds) and denervated (rectangles) groups are significantly different at the time point denoted, dagger  P < 0.05.



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Fig. 4.   Total immunoreactive Akt-alpha protein in RTA muscle from 24-h-denervated rats. Values are means ± SE; * P < 0.05, control vs. denervated (n = 6/group).

Total GLUT-4 and Translocated Surface GLUT-4

The level of total GLUT-4 was comparable in denervated and control soleus muscle (P > 0.05) (Fig. 5A). In a separate experiment, total GLUT-4 in RTA muscle was also not different in denervated muscle compared with control muscle (data not shown). The increased surface GLUT-4 availability after insulin stimulation (translocated GLUT-4) was determined by a trypsin cleavage method (Fig. 5B). Insulin increased surface GLUT-4 1.8-fold (P < 0.05) in control muscle, whereas no change in surface GLUT-4 was detected in denervated muscle (Fig. 5C).


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Fig. 5.   Trypsin-accessible surface GLUT-4 and total GLUT-4 in soleus muscle from 24-h-denervated rats. A: total GLUT-4 in control (C) and denervated (D) soleus muscles; n = 5 (P > 0.05). B: the trypsin cleavage method determines the effect of insulin on surface GLUT-4 availability (see METHODS). Anti-GLUT-4 immunoreactive bands at ~46 kDa represent native GLUT-4 (overexposed bands); bands at 35 kDa represent surface (trypsin-accessible) GLUT-4 in muscles from control animals (basal, lanes 1-3; insulin, lanes 4-6). Trypsin-accessible GLUT-4 was significantly increased (+97%) by insulin (P < 0.05). C: Means ± SE of scanned trypsin-cleaved bands. * P < 0.05 basal vs. insulin; n = 8-10 soleus strips for basal and insulin-treated muscle from denervated and control legs.


    DISCUSSION
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ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

This study has shown that insulin-stimulated glucose transport is decreased (Fig. 1) in short-term (24-h)-denervated muscle. Consistent with previous work (4, 12), this decrease is not due to a reduction in total GLUT-4; instead, we attribute denervation-induced insulin resistance to an impairment in Akt-alpha activation that is associated with a concomitant reduction in insulin-stimulated GLUT-4 translocation.

Insulin activates Akt-alpha through earlier signals that first activate PI 3-kinase (9). Increasing Akt-alpha kinase activity in vivo is accomplished by the phosphatidylinositol-dependent kinases PDK1 and PDK2 (13). PDK1 and PDK2 are proposed to phosphorylate Thr308 and Ser473, respectively, on Akt-alpha (see review by Coffer et al., Ref. 13). Intravenous insulin injections increase Akt-alpha kinase activity in rat muscle until maximum activity is seen after 5 min (48). In our studies, maximal Akt-alpha kinase activity was observed after 3 min in control muscle (data not shown).

Recently, Akt-alpha activation has been implicated as a key step in insulin signaling in skeletal muscle (32, 33, 42). Two studies (32, 42) have shown that the decrease in insulin-induced Akt-alpha activity and glucose transport was due to a prevailing hyperglycemia, because both Akt-alpha activity and glucose transport were restored to control levels once blood glucose was normalized. Moreover, decreases in Akt activity and glucose transport activation, reportedly caused by hyperglycemia (34), may be due to changes in insulin-signaling events downstream from PI 3-kinase. Kurowski et al. (34) showed that, with hyperglycemia, insulin-stimulated glucose transport is reduced in skeletal muscle (-30%). In these muscles, phosphotyrosine-immunoprecipitated PI 3-kinase activity and both IRS1- and IRS2-associated PI 3-kinase activity were not altered, whereas marked reductions in Akt activation (-60%) were observed. Therefore, insulin resistance in skeletal muscle, in some instances (i.e., during hyperglycemia), may be a consequence of reducing the signals from Akt.

Similar to hyperglycemia, the insulin resistance induced by denervation may involve a defect beyond PI 3-kinase. In previous studies, there appeared to be no defects in insulin signaling pathways (up to PI 3-kinase) in short-term (24-h)-denervated muscles (11, 19, 50). Our earlier data support the idea that PI 3-kinase activation occurs normally in muscles denervated for 24 h, because we found that the amount of insulin-stimulated PI 3-kinase regulatory subunit (p85) immunoprecipitated by antiphosphotyrosine antibodies was not decreased by denervation (50). However, our data here show that muscle denervation decreased insulin-stimulated Akt-alpha kinase activity (Fig. 2). This decrease is attributable to an impaired Akt-alpha activation. Denervation decreased the rate (-36%) of muscle Akt-alpha phosphorylation (Fig. 3) and muscle Akt-alpha protein content (-37%; Fig. 4). In contrast, Turinsky and Damrau-Abney (47) did not observe any changes in insulin-stimulated Akt-alpha kinase activity in short-term (24-h)-denervated muscles. We have no explanation for the discrepancy between our studies and their work. Nevertheless, based on our work, it may be that an inadequate insulin signaling response through Akt-alpha may contribute to the insulin resistance in 24-h-denervated muscles.

The lower insulin-stimulated Akt-alpha kinase activity induced by denervation could have occurred if impairments to the insulin signaling mechanism, before Akt-alpha in the insulin signaling pathway, contributed to impairments in Akt-alpha activation in denervated muscle. But because IR bind insulin with a normal affinity (8), and denervation does not appear to affect insulin-activated IR tyrosine kinase activity 24 h after muscle denervation (19), it is unlikely that the reduced Akt-alpha activation in 24-h-denervated muscle was due to poor IR activation. Moreover, our data show that supraphysiological plasma insulin concentrations did not further increase Akt-alpha kinase activity in the denervated muscle (Fig. 2); rather, the reductions in Akt-alpha kinase activity (~25% lower) were similar at both high physiological and supraphysiological insulin concentrations. Thus the lower insulin-stimulated Akt-alpha activation response in the denervated muscle was more likely due to a signaling change at a post-IR level rather than from insufficient IR tyrosine kinase activation.

Another possibility is that denervations had uncoupled upstream signals from Akt-alpha that would impair the ability of insulin to activate Akt-alpha in vivo. Because insulin-stimulated PI 3-kinase activity is not reported to be affected in muscle denervated for 24 h (11, 19, 50), PI 3-kinase-activated signals required to activate Akt-alpha in vivo were likely sufficient in denervated muscles. Furthermore, PDK2 was unlikely to be affected by denervation, because Akt-alpha [Ser473] phosphorylation still occurred in denervated muscle, albeit at a slower rate (Fig. 3). It is more likely that reductions in insulin-stimulated Akt-alpha kinase activity observed in 24-h-denervated muscle were a consequence of lower Akt-alpha protein availability in these muscles (Fig. 4). Interestingly, Akt-alpha [Ser473] phosphorylation in denervated muscle reached control levels by 5 min. Because phosphorylation on Thr308 also contributes to Akt-alpha activation (1), and both the Ser473 and Thr308 sites require phosphorylation to obtain full Akt-alpha kinase activation (1), our data suggest that a diminished Thr308 phosphorylation on Akt-alpha may account for the reduced Akt-alpha kinase activation in 24-h-denervated muscles.

Conceivably, an impairment in Akt-alpha activation in denervated muscles may attenuate signals that normally induce GLUT-4 translocation. Notably, there was a discrepancy in the percent reduction between insulin-induced Akt-alpha activation (-25%) and GLUT-4 translocation. It is possible that additional insulin-activated mechanisms, such as GLUT-4 trafficking or GLUT-4 membrane insertion steps, were also affected by denervation.

The reduction in glucose transport in denervated muscle was less than would have been expected, given that GLUT-4 at the surface was not increased by insulin. Undoubtedly, reduced GLUT-4 translocation contributed to insulin resistance in our studies. A number of studies have shown that glucose transport into the cell occurs in direct proportion to the surface GLUT-4 (35, 36). However, insulin may also stimulate glucose transport without necessarily increasing the number of surface GLUT-4 transporters (43). A variety of more recent studies have revitalized the idea that the intrinsic activity of surface GLUT-4 can be increased or decreased (17, 20, 22, 24, 40, 43). We have published work (22) showing that epinephrine can markedly lower glucose transport despite an insulin-induced increase in plasma membrane GLUT-4.

We believe that the results obtained in the soleus and RTA muscles with different insulin administration procedures can be integrated. It has previously been shown that the soleus and plantaris muscles, with markedly different fiber types, respond similarly to denervation (48). Two highly oxidative muscles such as the soleus and RTA would therefore be expected to respond similarly to denervation. Moreover, both muscles here were exposed to maximally stimulating concentrations of insulin either in vitro (soleus) or in vivo (RTA). Nevertheless, it is recognized that the delivery of insulin differs in vivo and in vitro. Therefore, the extent of GLUT-4 translocation or signaling per gram of muscle may be different between these two means of providing insulin.

In summary, our data demonstrate that there is a partial reduction in insulin-stimulated glucose transport along with a similar partial reduction in insulin-stimulated Akt-alpha activity in denervated muscle. These data indicate that short-term (24-h) denervation decreased Akt-alpha activation and impaired insulin-stimulated GLUT-4 appearance at the muscle cell surface.


    ACKNOWLEDGEMENTS

These studies were supported by the Canadian Diabetes Association in honor of George Goodwin and by the Natural Sciences and Engineering Research Council of Canada.


    FOOTNOTES

Address for reprint requests and other correspondence: A.Bonen, Dept. of Kinesiology, Univ. of Waterloo, Waterloo, Ontario N2L 3G1 Canada (E-mail: abonen{at}healthy.uwaterloo.ca).

The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.

Received 13 December 1999; accepted in final form 16 May 2000.


    REFERENCES
TOP
ABSTRACT
INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

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