Department of Physiology, College of Medicine, The Catholic University of Korea, Socho-gu, Seoul 137-701, Korea
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ABSTRACT |
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The action of tyrphostin AG-1478, a potent
protein tyrosine kinase (PTK) inhibitor, on rat brain Kv1.5 channels
(Kv1.5) stably expressed in Chinese hamster ovary cells was
investigated using the whole cell patch-clamp technique. AG-1478
rapidly and reversibly inhibited Kv1.5 currents at 50 mV in a
concentration-dependent manner with an IC50 of 9.82 µM.
AG-1478 accelerated the decay rate of inactivation of Kv1.5 currents
without modifying the kinetics of current activation. Pretreatment with
the structurally dissimilar PTK inhibitors (genistein and lavendustin
A) had no effect on the AG-1478-induced inhibition of Kv1.5 and did not
modify the AG-1478-induced current kinetics. The rate constants for
binding and unbinding of AG-1478 were 1.46 µM1 · s
1 and 10.19 s
1, respectively. The AG-1478-induced inhibition of Kv1.5
channels was voltage dependent, with a steep increase over the voltage range of channel opening. However, the inhibition exhibited voltage independence over the voltage range in which channels are fully activated. AG-1478 produced no significant effect on the steady-state activation or inactivation curves. AG-1478 slowed the deactivation time
course, resulting in a tail crossover phenomenon. Inhibition of Kv1.5
by AG-1478 was use dependent. The present results suggest that AG-1478
acts directly on Kv1.5 currents as an open-channel blocker and
independently of the effects of AG-1478 on PTK activity.
open-channel block
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INTRODUCTION |
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THE MODULATION OF ION
CHANNELS by the activation of protein kinases linked to
second-messenger systems has been studied extensively (16,
19). A number of protein kinase inhibitors have been widely used
to investigate the role of protein kinases in the modulation pathways
of ion channels. However, recent studies have indicated that protein
kinase inhibitors have a direct action on voltage- or ligand-gated ion
channels and ion transporters in a phosphorylation-independent manner.
Of the protein kinase C (PKC) inhibitors, calphostin C and
staurosporine have been shown to directly block L-type Ca2+
channels (12) and muscarinic K+ channels
(21), respectively. H-89, a protein kinase A (PKA) inhibitor, also has nonspecific and direct inhibitory effects on
sarcoplasmic reticulum Ca2+-ATPase (15).
Although protein phosphorylation by serine/threonine kinases is a major
mechanism for the modulation of ion channels, tyrosine kinases also
play an important role in regulating the activity of several ion
channels (9, 16, 23). Similarly, a protein tyrosine kinase
(PTK) inhibitor, genistein, directly inhibits glycine receptors
(14) and K+ currents (25, 33) and
directly activates Cl channels (34). The
possibility that genistein and tyrphostin 23 directly block
Ca2+ channels has also been discussed (4, 36).
In previous studies (5, 8), we found that staurosporine and another PKC inhibitor, bisindolylmaleimide, directly block cloned Kv1.3 and Kv1.5, respectively, without mediation through PKC inhibition or any diffusible cytosolic molecules. Recently, we have also reported (7) that H-89 inhibits cloned Kv1.3 channels independently of PKA-involved pathways. Therefore, we designed the present study to investigate the direct action of tyrphostin AG-1478, a PTK inhibitor, on cloned rat Kv1.5 expressed in Chinese hamster ovary (CHO) cells by using the whole cell patch-clamp technique.
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MATERIALS AND METHODS |
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Stable transfection and cell culture. Rat brain Kv1.5 channels stably expressed in CHO cells (5) were used in the present study. The method for establishing Kv1.5 expression in CHO cells is briefly described as follows. Kv1.5 cDNA (29) was transferred into the plasmid expression vector pCR3.1 (Invitrogen, San Diego, CA). CHO cells were stably transfected with Kv1.5 cDNA by using FuGENE 6 reagent (Boehringer Mannheim, Indianapolis, IN). The transfected cells were cultured in Iscove's modified Dulbecco's medium (IMDM; Life Technologies, Grand Island, NY) supplemented with 10% fetal bovine serum, 2 mM glutamine, 0.1 mM hypoxanthine, 0.01 mM thymidine, and 0.2 mg/ml G418 (Life Technologies) under a 95% humidified air-5% CO2 environment at 37°C. The cultures were passaged every 4-5 days by using a brief trypsin-EDTA treatment. The trypsin-EDTA-treated cells were seeded onto glass coverslips (diameter 12 mm; Fisher Scientific, Pittsburgh, PA) in a petri dish. After 12-24 h, the cell-attached coverslips were transferred to a continually perfused recording chamber (RC-13; Warner Instrument, Hamden, CT) for electrophysiological recordings.
Electrophysiological recordings.
At room temperature (22-24°C), whole cell currents of Kv1.5 were
recorded and stored by using the patch-clamp technique
(11) with an Axopatch 1D amplifier (Axon Instruments,
Foster City, CA) and an IBM compatible computer equipped with a
Digidata 1200A acquisition board (Axon Instruments). Currents were
sampled at 5 kHz and filtered at 2 kHz (4-pole Bessel filter). Pulse
generation and data acquisition were controlled with pCLAMP 6.05 software (Axon Instruments). Patch electrodes were fabricated by using PG10165-4 glass capillary tubing (World Precision Instruments, Sarasota, FL). Liquid junction potentials between external and pipette
solutions were offset. Whole cell capacitive current compensation and
80% series resistances compensation were done without any leakage
compensation. Whole cell currents of ~1-4 nA and series resistances of 2-3 M were used for the analysis.
Solutions and drugs. The pipette solution contained (in mM) 140 KCl, 1 CaCl2, 1 MgCl2, 10 HEPES, and 10 EGTA and was adjusted to pH 7.3 with KOH. The bath solution contained (in mM) 140 NaCl, 5 KCl, 1.3 CaCl2, 1 MgCl2, 20 HEPES, and 10 glucose and was adjusted to pH 7.3 with NaOH. Genistein (10 µM; Calbiochem, San Diego, CA) was added to the bath solution for genistein-pretreatment experiments. Genistein, lavendustin A (Calbiochem), and AG-1478 (Sigma Chemical, St. Louis, MO) were dissolved in dimethyl sulfoxide (DMSO; Sigma Chemical) to yield stock solutions of 50 mM. The concentration of DMSO in the final solution was <0.1% and had no effect on Kv1.5 currents.
Data analysis.
Data were analyzed by using pCLAMP 6.05 (Axon Instruments) and Origin
6.1 software (Microcal Software, Northampton, MA). The concentration-dependent curve for current inhibition by AG-1478 was
fitted to the Hill equation
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(1) |
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(2a) |
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(2b) |
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(3) |
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(4) |
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RESULTS |
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Reversible and direct inhibition.
As shown in Fig. 1A, Kv1.5
currents were rapidly activated under control conditions, with a
sigmoidal time course rising to a peak, and then slowly inactivated, as
reported previously (5). The dominant time constant of
activation under control conditions was 1.46 ± 0.23 ms
(n = 5) with a 250-ms depolarizing pulse from 80 to
50 mV. In the presence of 10 µM AG-1478, the time constant of
activation was 1.42 ± 0.21 ms (n = 6), which
indicates that the activation kinetics were not significantly modified
by AG-1478. In the presence of AG-1478, the current was initially
activated as under control conditions without significantly affecting
the peak current amplitude. However, the slow inactivation was markedly accelerated, resulting in an apparent decrease in the steady-state current amplitude at the end of a 250-ms depolarizing pulse. Thus AG-1478 (10 µM) inhibited the steady-state current of Kv1.5 at the
end of a depolarizing pulse of 50 mV by 54.9 ± 1.7% of the control value (n = 6). As shown in Fig. 1B,
repeated bath perfusions of 10 µM AG-1478 induced a rapid and
reversible inhibition of Kv1.5 currents at the end of a 250-ms
depolarizing pulse. Kv1.5 inhibition appeared within 20 s of the
drug being applied and reached a steady state within 3 min. The effect
of AG-1478 was largely reversed following a 2-min washout. Under
control conditions without AG-1478, the peak amplitude of Kv1.5
currents did not display any noticeable change over a 20-min period
(90.0 ± 2.5% of the control value, n = 5).
Although the current was not completely restored after washout of the
drug (86.1 ± 1.3% of control values, n = 8), the
degree of inhibition was not affected by repeated treatment with the
drug. A comparison plot of the time course of Kv1.5 currents for an
individual experiment in the absence and presence of AG-1478 is shown
in Fig. 1B.
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Concentration-dependent inhibition.
Figure 3A shows superimposed
Kv1.5 current traces produced by a 250-ms depolarizing pulse to 50 mV
under control conditions and in the presence of various concentrations
of AG-1478. AG-1478 induced a reduction in the steady-state current
amplitude during the depolarizing pulse, with little effect on the peak
amplitude. The current amplitude measured at the end of a 250-ms
depolarizing pulse was used as an index of inhibition. A nonlinear
least-squares fit of the Hill equation (Eq. 1) to the
individual data points yielded an IC50 value of 9.82 ± 1.07 µM and a Hill coefficient of 1.45 ± 0.23 (Fig.
3B, n = 6). Furthermore, AG-1478 induced a
concentration-dependent increase in the rate of current decay. The
traces of current decay at each concentration (10, 30, and 100 µM) of
AG-1478 were well fitted to a single exponential function, which
yielded a time constant (D) for Kv1.5 current
inhibition. Because the Kv1.5 channel undergoes an intrinsic slow
inactivation during the depolarizing pulse, we disregarded the time
constant values at low concentrations (3 µM) of AG-1478 in
calculating a good approximation of the time constant for the
development of drug-induced inhibition. Plotting
D at 50 mV against each concentration and fitting it to a hyperbolic equation
(Eq. 2a) yielded binding (k1) and
unbinding rate constants (k
1) of 1.46 ± 0.06 µM
1 · s
1 and 10.19 ± 2.79 s
1, respectively (Fig. 3C). The
theoretical Kd value derived by k
1/k1 (Eq. 2b) was 6.98 µM. Although the derived Kd
of 6.98 µM is independent of the IC50 of 9.82 µM
calculated from the concentration-response curve (Fig. 3B),
the two values are reasonably close, suggesting that AG-1478-induced
inhibition of the Kv1.5 channel follows a simple one-to-one reaction.
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Voltage dependence of inhibition.
Figure 4A shows the
current-voltage (I-V) relations obtained under control
conditions and in the presence of 10 µM AG-1478. Under control
conditions, the Kv1.5 current was activated at pulses greater than 30
mV. The I-V relationship shows a sigmoidal shape at
potentials between
30 and 0 mV and is almost linear for depolarizing pulses >0 mV. In the presence of 10 µM AG-1478, an inhibition of
steady-state currents was observed through the whole voltage range over
which Kv1.5 was activated. When the relative current (IAG-1478/Icontrol)
was plotted against the membrane potential (Fig. 4B), a high
degree of inhibition with strong voltage dependence was observed
between
30 and 0 mV, which corresponds to the voltage range of
channel opening. This suggests that AG-1478-induced inhibition of Kv1.5
currents occurs preferentially after channels open. However, the
inhibition of Kv1.5 channels by AG-1478 in the range of voltages between 0 and 50 mV, where channels are fully activated, did not show
voltage dependence. The linear curve fitting of the data at potentials
>than 0 mV (Fig. 4B, solid line) yielded a value approximately equal to zero for the slope of the line: 51.3 ± 3.4% of the control value at 0 mV and 49.6 ± 2.2% of the
control at 50 mV (n = 4, ANOVA, P > 0.05). The voltage dependence of the inactivation time constant (
)
is plotted in Fig. 4C. The inactivation time course under
control conditions was not significantly altered (ANOVA,
P > 0.05) at different voltages between 20 and 50 mV. The time constants of the decay phase of Kv1.5 were significantly reduced in the presence of 10 µM AG-1478 (111-137 ms for the
control; 41-44 ms for AG-1478), but this effect did not vary over
the voltage range examined (ANOVA, P > 0.05).
Therefore, these results indicate that AG-1478-induced inhibition of
Kv1.5 channels is voltage-independent over the voltage range in which
channels are fully activated.
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Voltage dependence of the steady-state activation and inactivation.
We examined whether AG-1478 affected Kv1.5 by shifting the steady-state
activation curve (Fig. 5A). A
two-pulse protocol was used to estimate the voltage dependence of Kv1.5
by tail current (Fig. 5A, top) analysis in the
absence and presence of AG-1478. As shown in Fig. 5A, the
activation curve was unchanged in the presence of 10 µM AG-1478. The
potential of half-maximum activation (V1/2) was
11.08 ± 1.23 and
13.85 ± 0.92 mV before and after application of the drug (n = 4). Similarly, there was
no significant shift in slope value (k) in the presence of
AG-1478 (5.95 ± 0.67 mV for control; 7.39 ± 0.50 mV for
AG-1478, n = 4). To further examine the possibility of
inhibition in the inactivated state, we analyzed the effect of AG-1478
on the steady-state inactivation of Kv1.5. Figure 5B,
top, shows control currents and those in the presence of 10 µM
AG-1478. In the absence of drug, the midpotential (V1/2) and slope value (k) of the
steady-state inactivation curve measured
23.72 ± 0.32 and
6.19 ± 0.28 mV, respectively (Fig. 5B,
bottom). After the addition of 10 µM AG-1478, the
V1/2 and k showed no change,
measuring
25.86 ± 0.13 and 6.48 ± 0.11 mV, respectively.
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Effects of AG-1478 on the deactivation kinetics of Kv1.5.
To further investigate the voltage dependence of inhibition, the
effects of AG-1478 on the Kv1.5 current deactivation kinetics were
studied. Figure 6, A and
B, shows the representative superimposed tail currents
recorded with a 250-ms repolarizing pulse between 50 and
20 mV
after a 250-ms depolarizing pulse of 50 mV from a holding potential of
80 mV under control conditions and in the presence of 10 µM
AG-1478. These currents were well fitted to a single exponential
function. Under control conditions, the tail current declined with a
time constant of 22.19 ± 1.54 ms (n = 4) and was
nearly completely deactivated during a 250-ms repolarizing pulse of
40 mV. In the presence of 10 µM AG-1478, the initial peak amplitude
of the tail current was reduced and the subsequent decline of the
current was slowed (57.16 ± 2.11 ms, n = 4, Student's t-test, P < 0.05), which
resulted in a crossover phenomenon (Fig. 6C). Figure
6D shows a summary of the time constants calculated for
repolarizing pulses between
50 and
20 mV in the absence and
presence of AG-1478. Although AG-1478 significantly increased the
deactivation time constants of Kv1.5 over the voltage range tested, the
differences between the time constants obtained from tail currents in
the absence and presence of AG-1478 did not change (ANOVA, P
> 0.05). These results provide further evidence of the voltage
independence of inhibition of Kv1.5 by AG-1478.
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Use-dependent inhibition.
Original current traces, under control conditions and in the presence
of 10 µM AG-1478, were produced by 15 repetitive applications of
depolarizing pulses at two different frequencies, 1 and 2 Hz (Fig.
7A). Under control conditions,
the peak amplitude of the Kv1.5 current decreased slightly by 9.0 ± 0.5% (n = 4) at a frequency of 1 Hz and by
16.1 ± 0.9% (n = 4) at a frequency of 2 Hz (Fig. 7B). In the presence of 10 µM AG-1478, the peak amplitude
of Kv1.5 was not significantly reduced after the first pulse. The
subsequent trace showed a progressive decrease in the peak amplitude of
Kv1.5 to a steady level. The extent of the steady-state inhibition was 28.5 ± 0.8% (n = 4) and 42.7 ± 1.5%
(n = 4) at 1 and 2 Hz, respectively. Thus AG-1478 exhibited
use-dependent inhibition of Kv1.5 with little tonic block.
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The membrane sidedness of AG-1478 action on Kv1.5. To study the possibility that AG-1478 acts at an intracellular site, AG-1478 (100 µM) was included in the pipette solution. After the whole cell configuration was established, the magnitude and kinetics of Kv1.5 were found to be unaltered compared with control experiments (92.0 ± 1.4% of control value, n = 4) over a 10-min period. Subsequent external application of 10 µM AG-1478 promptly inhibited Kv1.5 currents (51.3 ± 2.1% of control value, n = 4). These results suggest that the site of action of AG-1478 on this channel is accessible from outside the membrane.
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DISCUSSION |
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The present study shows that AG-1478, a tyrosine kinase inhibitor, reversibly inhibits the steady-state current of Kv1.5 in a concentration-, time-, and use-dependent manner. These results suggest that this inhibitory action is not mediated via the inhibition of PTK but is probably due to a direct interaction of the drug with Kv1.5.
It is well known that the activity of K+ channels can be modulated by phosphorylation and dephosphorylation (9, 16, 19, 23). Consensus protein phosphorylation sites are prevalent in the deduced amino acid sequences of cloned Kv1.5 channel proteins (29, 32). Although tyrosine phosphorylation can play an important role in regulating long-term changes in cellular functions such as cell growth and differentiation, recent studies have shown that tyrosine phosphorylation can rapidly modulate native and cloned ion channels, including ligand-gated and voltage-gated channels (3, 9, 13, 23, 31).
AG-1478 is a potent inhibitor of PTK and has been used to investigate the physiological role of PTK in the regulation of various cellular functions (20). However, our results suggest that the effects of AG-1478 on Kv1.5 do not occur via phosphotyrosine-signaling pathways for the following five reasons.
First, Kv1.5 inhibition was detected rapidly within 20 s of the application of the drug and reached a steady state within 3 min. Its effect was also highly reversible within 2 min. The activation of receptor or nonreceptor PTKs, which results in the tyrosine phosphorylation of ion channels, modulates channel activity with a relatively slow time course of inhibition. The amplitude of Kv1.5 currents, for example, is modified by tyrosine phosphorylation with a half-time of ~20 min, and the recovery of the suppressed current is much slower than the initial rate of decline (30). Furthermore, there is a delay of a few minutes before any changes in current amplitude are detected. Therefore, the slow time course of channel modulation by PTK inhibition in other studies (24, 30) does not parallel the time course of the inhibition of Kv1.5 currents in our study. We conclude, therefore, that the rapid time course taken to reach the steady-state inhibition of Kv1.5 by AG-1478 and the rapid time course of the reverse reaction are not simply explicable by the inhibition of PTK activity.
Second, pretreatment with the structurally dissimilar PTK inhibitors genistein and lavendustin A had no effect on the AG-1478-induced inhibition of Kv1.5 and did not modify the AG-1478-induced current kinetics. Because genistein and lavendustin A are potent PTK inhibitors (2, 20) and are structurally different from AG-1478, these results suggest that AG-1478 directly interacts with Kv1.5 without the mediation of PTK inhibition. Furthermore, in the present study, AG-1478-induced inhibition of Kv1.5 was observed under nonphosphorylating conditions (no ATP in the pipette solution). Although AG-1478 is selective for tyrosine kinases, it is still possible that AG-1478 can also nonspecifically block other types of protein kinases, such as PKC (20). Although we cannot completely exclude the possibility that the inhibition of Kv1.5 results indirectly from the inhibition of PKC, our previous studies showed that PKC inhibition has no effect on Kv1.5 (5, 6).
Third, although the down- or upregulation of Kv1.5 by PTK is controversial (13, 22, 30), the observed pattern of Kv1.5 current suppression by tyrosine phosphorylation is completely different from the AG-1478-induced inhibition in the present case. Inhibition by tyrosine phosphorylation has been characterized by a slowing of the apparent rate of activation (13) and by a reduction in peak current amplitude, whereas inactivation acceleration has not been detected (13, 30). Furthermore, Kv1.3 and Kv3.1 channels have been shown to be inhibited by protein phosphorylation over a long time course with no changes in inactivation kinetics (1, 17). In the present study, time-dependent decay of the current was evident in the presence of AG-1478, reflecting different kinetics of inhibition for AG-1478.
Fourth, AG-1478 did not influence the kinetics of activation or the voltage dependence of the steady-state activation and inactivation curves of Kv1.5 currents. The major point made by the present work is that time-dependent inhibition of Kv1.5 occurred in the presence of AG-1478. Its inhibitory action is characterized by an acceleration of current inactivation. Moreover, AG-1478 decreases the rate of decay of the tail current and induces a crossover phenomenon. These results suggest that the inhibition of Kv1.5 by AG-1478 can be described in terms of an open-channel blocking mechanism (10). The effects of AG-1478 were also use dependent: the degree of current inhibition increased with repetitive depolarizations. Furthermore, in the presence of AG-1478, the peak amplitude of Kv1.5 currents was not significantly reduced after the first pulse, suggesting no tonic block by the drug. These phenomena are also cited as evidences of an open-channel blocking mechanism. Taken together, these results indicate that AG-1478 directly blocks the open-state Kv1.5 channel as a pore blocker and probably accesses its binding site from the extracellular face of the channel.
Fifth, a high degree of inhibition with strong voltage dependence was observed in the voltage range of channel opening. These results suggest that AG-1478 preferentially binds to the open state of the channel. The blocking effects of AG-1478 resemble those previously described for other positively-charged drugs (26). In contrast to the block produced by these drugs, however, AG-1478-induced inhibition of the Kv1.5 channel was voltage-independent over the voltage range at which channels are fully activated (Fig. 4, B and C). Further evidence of the voltage independence of Kv1.5 inhibition is the observation that AG-1478 had no effect on the voltage dependence of current deactivation kinetics as determined from tail currents (Fig. 6D). Because AG-1478 (pKa = 6.5) is mainly in the uncharged form at the intracellular pH of 7.3 (pH of the pipette solution), the interaction between the uncharged AG-1478 molecule and the Kv1.5 channel is not affected by the transmembrane electrical field. Therefore, the voltage independence could be explained in one of three ways. First, even if the drug did enter from the outside and bind deeply within the transmembrane electrical field, there could be little or no voltage dependence to its binding, given the uncharged nature of AG-1478. Another possibility is that the binding site for AG-1478 does not lie within the channel pore and therefore is exposed only slightly to the membrane electrical field. However, an alternative explanation for voltage independence could be that AG-1478 interacts with the Kv1.5 channel by a hydrophobic pathway, altering the inactivation kinetics by an allosteric mechanism. In general, the voltage dependence of an open-channel block is produced by the charged form of the drugs. Therefore, it is difficult to understand the nature of such a blocking mechanism. However, an open-channel block produced by the uncharged form of a drug has also been observed (5, 6, 18, 37).
We cannot completely rule out the possibility that the inhibition of Kv1.5 by AG-1478 results indirectly through the inhibition of unidentified signal transduction pathways. However, taking all these results together, we infer that the inhibition of Kv1.5 currents by AG-1478 does not occur via a phosphorylation-dependent signal transduction pathway but via a direct one-to-one interaction between the drug and the channel in the open state.
In conclusion, the present study shows that AG-1478, a potent PTK inhibitor, is able to inhibit cloned rat brain Kv1.5 channels independently of PTK activity. On the basis of the present study and considering previous reports demonstrating the mechanisms of the direct actions of PKC, PKA, and PTK inhibitors on ion channels (4, 5, 7, 8, 14, 21, 25, 33, 34), we recommend caution in the use of these kinds of drugs in physiological experiments designed to determine the role of protein kinases in the modulation of ion channels. Alternatively, this study provides a pharmacological tool for the development of a specific ion-channel blocker.
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ACKNOWLEDGEMENTS |
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We thank Dr. Leonard Kaczmarek (Yale University School of Medicine) for the rat Kv1.5 cDNA.
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FOOTNOTES |
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This work was supported by Korea Research Foundation Grant KRF-2001-041-F00043.
Address for reprint requests and other correspondence: S. J. Hahn, Dept. of Physiology, College of Medicine, The Catholic Univ. of Korea, 505 Banpo-dong, Socho-gu, Seoul 137-701, Korea (E-mail: sjhahn{at}cmc.cuk.ac.kr).
The costs of publication of this article were defrayed in part by the payment of page charges. The article must therefore be hereby marked "advertisement" in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.
First published February 20;10.1152/ajpcell.00398.2001
Received 14 August 2001; accepted in final form 28 January 2002.
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REFERENCES |
---|
![]() ![]() ![]() ![]() ![]() ![]() ![]() |
---|
1.
Aiyar, J,
Grissmer S,
and
Chandy KG.
Full-length and truncated Kv1.3 K+ channels are modulated by 5-HT1c receptor activation and independently by PKC.
Am J Physiol Cell Physiol
265:
C1571-C1578,
1993
2.
Akiyama, T,
Ishida J,
Nakagawa S,
Ogawara H,
Watanabe S,
Itoh N,
Shibuya M,
and
Fukami Y.
Genistein, a specific inhibitor of tyrosine-specific protein kinases.
J Biol Chem
262:
5592-5595,
1987
3.
Boxall, AR,
and
Lancaster B.
Tyrosine kinases and synaptic transmission.
Eur J Neurosci
10:
2-7,
1998[ISI][Medline].
4.
Chiang, CE,
Chen SA,
Chang MS,
Lin CI,
and
Luk HN.
Genistein directly inhibits L-type calcium currents but potentiates cAMP-dependent chloride currents in cardiomyocytes.
Biochem Biophys Res Commun
223:
598-603,
1996[ISI][Medline].
5.
Choi, BH,
Choi JS,
Jeong SW,
Hahn SJ,
Rhie DJ,
Jo YH,
and
Kim MS.
Direct block by bisindolylmaleimide of rat Kv1.5 expressed in Chinese hamster ovary cells.
J Pharmacol Exp Ther
293:
634-640,
2000
6.
Choi, BH,
Choi JS,
Min DS,
Yoon SH,
Rhie DJ,
Jo YH,
Kim MS,
and
Hahn SJ.
Effects of ()-epigallocatechin-3-gallate, the main component of green tea, on the cloned rat brain Kv1.5 potassium channels.
Biochem Pharmacol
62:
527-535,
2001[ISI][Medline].
7.
Choi, JS,
Choi BH,
Hahn SJ,
Yoon SH,
Min DS,
Jo YH,
and
Kim MS.
Inhibition of Kv1.3 channels by H-89 (N-[2-(p-bromocinnamylamino)ethyl]-5-isoquinolinesulfonamide) independent of protein kinase A.
Biochem Pharmacol
61:
1029-1032,
2001[ISI][Medline].
8.
Choi, JS,
Hahn SJ,
Rhie DJ,
Jo YH,
and
Kim MS.
Staurosporine directly blocks Kv1.3 channels expressed in Chinese hamster ovary cells.
Naunyn Schmiedebergs Arch Pharmacol
359:
256-261,
1999[ISI][Medline].
9.
Fadool, DA,
and
Levitan IB.
Modulation of olfactory bulb neuron potassium current by tyrosine phosphorylation.
J Neurosci
18:
6126-6137,
1998
10.
Franqueza, L,
Valenzuela C,
Delpon E,
Longobardo M,
Caballero R,
and
Tamargo J.
Effects of propafenone and 5-hydroxy-propafenone on hKv1.5 channels.
Br J Pharmacol
125:
969-978,
1998[Abstract].
11.
Hamill, OP,
Marty A,
Neher E,
Sakmann B,
and
Sigworth FJ.
Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches.
Pflügers Arch
391:
85-100,
1981[ISI][Medline].
12.
Hartzell, HC,
and
Rinderknecht A.
Calphostin C, a widely used protein kinase C inhibitor, directly and potently blocks L-type Ca channels.
Am J Physiol Cell Physiol
270:
C1293-C1299,
1996
13.
Holmes, TC,
Fadool DA,
Ren R,
and
Levitan IB.
Association of Src tyrosine kinase with a human potassium channel mediated by SH3 domain.
Science
274:
2089-2091,
1996
14.
Huang, RQ,
and
Dillon GH.
Direct inhibition of glycine receptors by genistein, a tyrosine kinase inhibitor.
Neuropharmacology
39:
2195-2204,
2000[ISI][Medline].
15.
Hussain, M,
Drago GA,
Bhogal M,
Colyer J,
and
Orchard CH.
Effects of the protein kinase A inhibitor H-89 on Ca2+ regulation in isolated ferret ventricular myocytes.
Pflügers Arch
437:
529-537,
1999[ISI][Medline].
16.
Jonas, EA,
and
Kaczmarek LK.
Regulation of potassium channels by protein kinases.
Curr Opin Neurobiol
6:
318-323,
1996[ISI][Medline].
17.
Kanemasa, T,
Gan L,
Perney TM,
Wang LY,
and
Kaczmarek LK.
Electrophysiological and pharmacological characterization of a mammalian Shaw channel expressed in NIH 3T3 fibroblasts.
J Neurophysiol
74:
207-217,
1995
18.
Lacerda, AE,
Roy ML,
Lewis EW,
and
Rampe D.
Interactions of the nonsedating antihistamine loratadine with a Kv1.5-type potassium channel cloned from human heart.
Mol Pharmacol
52:
314-322,
1997
19.
Levitan, IB.
Modulation of ion channels by protein phosphorylation and dephosphorylation.
Annu Rev Physiol
56:
193-212,
1994[ISI][Medline].
20.
Levitzki, A,
and
Gazit A.
Tyrosine kinase inhibition: an approach to drug development.
Science
267:
1782-1788,
1995[ISI][Medline].
21.
Lo, CF,
and
Breitwieser GE.
Protein kinase-independent inhibition of muscarinic K+ channels by staurosporine.
Am J Physiol Cell Physiol
266:
C1128-C1132,
1994
22.
MacFarlane, SN,
and
Sontheimer H.
Modulation of Kv1.5 currents by Src tyrosine phosphorylation: potential role in the differentiation of astrocytes.
J Neurosci
20:
5245-5253,
2000
23.
Peretz, A,
Sobko A,
and
Attali B.
Tyrosine kinases modulate K+ channel gating in mouse Schwann cells.
J Physiol
519:
373-384,
1999
24.
Potier, B,
and
Rovira C.
Protein tyrosine kinase inhibitors reduce high-voltage activating calcium currents in CA1 pyramidal neurones from rat hippocampal slices.
Brain Res
816:
587-597,
1999[ISI][Medline].
25.
Smirnov, SV,
and
Aaronson PI.
Inhibition of vascular smooth muscle cell K+ currents by tyrosine kinase inhibitors genistein and ST 638.
Circ Res
76:
310-316,
1995
26.
Snyders, DJ,
Bennett PB,
and
Hondeghem LM.
Mechanisms of drug-channel interaction.
In: The Heart and Cardiovascular System (2nd ed.), edited by Fozzard H,
Haber E,
Jennings R,
and Katz A.. New York: Raven, 1992, p. 2165-2193.
27.
Snyders, DJ,
Tamkun MM,
and
Bennett PB.
A rapidly activating and slowly inactivating potassium channel cloned from human heart: functional analysis after stable mammalian cell culture expression.
J Gen Physiol
101:
513-543,
1993[Abstract].
28.
Snyders, DJ,
and
Yeola SW.
Determinants of antiarrhythmic drug action: electrostatic and hydrophobic components of block of the human cardiac hKv1.5 channel.
Circ Res
77:
575-583,
1995
29.
Swanson, R,
Marshall J,
Smith JS,
Williams JB,
Boyle MB,
Folander K,
Luneau CJ,
Antanavage J,
Oliva C,
Buhrow SA,
Bennett C,
Stein RB,
and
Kaczmarek LK.
Cloning and expression of cDNA and genomic clones encoding three delayed rectifier potassium channels in rat brain.
Neuron
4:
929-939,
1990[ISI][Medline].
30.
Timpe, LC,
and
Fantl WJ.
Modulation of a voltage-activated potassium channel by peptide growth factor receptors.
J Neurosci
14:
1195-1201,
1994[Abstract].
31.
Tsai, W,
Morielli AD,
and
Peralta EG.
The m1 muscarinic acetylcholine receptor transactivates the EGF receptor to modulate ion channel activity.
EMBO J
16:
4597-4605,
1997
32.
Tseng-Crank, JCL,
Tseng GN,
Schwartz A,
and
Tanouye MA.
Molecular cloning and functional expression of a potassium channel cDNA isolated from a rat cardiac library.
FEBS Lett
268:
63-68,
1990[ISI][Medline].
33.
Washizuka, T,
Horie M,
Obayashi K,
and
Sasayama S.
Genistein inhibits slow component delayed-rectifier K currents via a tyrosine kinase-independent pathway.
J Mol Cell Cardiol
30:
2577-2590,
1998[ISI][Medline].
34.
Weinreich, F,
Wood PG,
Riordan JR,
and
Nagel G.
Direct action of genistein on CFTR.
Pflügers Arch
434:
484-491,
1997[ISI][Medline].
35.
White, MM,
and
Bezanilla F.
Activation of squid axon K+ channels: ionic and gating current studies.
J Gen Physiol
85:
539-554,
1985[Abstract].
36.
Wijetunge, S,
Aalkjaer M,
Schachter M,
and
Hughes AD.
Tyrosine kinase inhibitors block calcium channel currents in vascular smooth muscle cells.
Biochem Biophys Res Commun
189:
1620-1623,
1992[ISI][Medline].
37.
Zhang, X,
Anderson JW,
and
Fedida D.
Characterization of nifedipine block of the human heart delayed rectifier, hKv1.5.
J Pharmacol Exp Ther
281:
1247-1256,
1997