1 Institute of Community Medicine, University of Tromsø, Tromsø, Norway.
2 Department of Mathematics, University of Bergen, Bergen, Norway.
3 Center for International Health, University of Bergen, Bergen, Norway.
Received for publication June 14, 2002; accepted for publication November 19, 2002.
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ABSTRACT |
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menopause; menstruation; mortality; postmenopause; prospective studies
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INTRODUCTION |
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In a 6-year follow-up of 5,200 Seventh-day Adventists, women with an early menopause (<40 years) had a total mortality rate twice that of women who experienced menopause when they were 5054 years of age (3). In an extended 12-year follow-up with a total of 1,831 deaths, the inverse relation between age at menopause and total mortality was confirmed, but the association was weakened (2).
In a recent study with 181 deaths among 1,462 naturally postmenopausal Belgian women, women with menopausal ages above 50 had a significantly reduced all-cause mortality compared with women with earlier menopause (10). Similarly, in a 4-year follow-up of individuals from the First National Health and Nutrition Examination Survey, it was found that US women who reported a natural menopause at age 39 years or below had 50 percent increased mortality compared with women who had their menopause when they were age 50 years or above. The linear trend with age at menopause was of borderline significance (p = 0.06). The results were, however, based on only 299 deaths, 24 of which occurred in women with a very early menopause (<40 years) (7). In a still smaller study (8), no significant relation was found between age at natural menopause and total mortality. However, because of the low number of deaths (a total of 82), the confidence intervals were wide.
Thus, at present, it is unclear whether age at natural menopause has any impact on total mortality. We have therefore investigated this relation in a Norwegian cohort including 19,731 women with 37 years of follow-up. During this period, 18,533 women died. The large number of deaths made possible detailed analyses within subgroups defined by attained age as well as possible confounders.
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MATERIALS AND METHODS |
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Information on height and weight was available for 13,622 of the 19,731 women, derived from separate measurements made during the period 19631975 as part of a compulsory mass examination for tuberculosis. Body mass index was defined as weight (kg)/height (m)2.
Associations between age at menopause and reproductive and demographic factors recorded before the start of follow-up were explored in analyses with age at menopause as the dependent variable. Possible predictors for age at menopause were entered as categorical factors in an analysis of variance.
The official personal registration number served as a unique identification of each woman and made it possible to link our data about age at menopause (which occurred sometime before the screening in 19561959) to information on vital status and cause of death obtained from files kept at Statistics Norway, Oslo. Follow-up started on January 1, 1961, when the personal registration number was introduced. During the complete follow-up through 1997, 18,533 women died.
The relations between age at natural menopause and the total mortality rate were investigated in a Cox proportional hazards regression model using attained age (divided into 2-week periods) as the time variable. Women who were alive at the end of follow-up were censored on December 31, 1997, and women who emigrated were censored on the day they left Norway. In all analyses, we adjusted for birth cohort (18861889, 18901894, 18951899, 19001904, 19051909, 19101914, 19151926), county of residence and, as an indicator of social class (12), occupational group (own or husbands occupation). Married housewives were assigned to the occupational class of their husbands.
Age at menopause was categorized into seven groups: aged 40 years or below, 4143, 4446, 4749, 5052, 5355, and 5660 years. Women aged 5052 years were considered the reference category. However, when computing the p value for linear trend and mean change in mortality rate related to an increase in age at menopause by 3 years, we included age at menopause in the model as a continuous (1-year interval) variable. Body mass index was adjusted for in separate analyses restricted to women with information about height and weight and with follow-up starting January 1 of the year after the measurements were made.
From previous analyses (1), we suspected that the effect of age at menopause on total mortality might be influenced by the attained age of the women. We therefore performed the analyses in three predetermined age groups: <70 years, 7079 years, and 80 years or above. In addition, other analyses were carried out within subgroups defined by demographic and reproductive variables.
All analyses were performed using SAS software (13).
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RESULTS |
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Occupational group, county, ever been married, age at menarche, and parity were considered possible predictors for age at natural menopause. After adjustment for birth cohort and the other predictors considered, only minor (although statistically significant (p 0.005)) differences remained in the mean age at natural menopause between women in the separate occupational groups and counties and between women with different ages at menarche or different parity. The maximum contrast was a 0.6-year lower age at menopause in nulliparous women than in women with five deliveries. Having ever been married had no bearing on the age at menopause (p = 0.99).
In an analysis confined to women with two or more deliveries, we also included parity and ages at first and last delivery as possible predictors. Ever been married was omitted as a possible predictor, however, as very few never married women had two or more children. Among the variables related to reproduction, only age at last delivery was significantly related to age at menopause, with an adjusted 0.8-year later menopause in women with an age at last delivery of 40 years or above compared with women with a relatively young age at last delivery of less than 28 years (p < 0.001). A statistically significant linear positive relation between age at menopause and age at last delivery was also found in women born before 1900, with a difference in mean age at menopause between the extreme groups (40 years vs. <28 years) of 1.3 years.
In this cohort, 18, 19, and 16 percent of the deaths were caused by ischemic heart disease, stroke, and cancer, respectively (table ). Other diseases caused 43 percent of the deaths, whereas violent deaths were rather uncommon. The cause of death was unknown for less than 1 percent of all deaths. This category occurred almost exclusively in the early parts of the follow-up, during 19611970.
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Mortality among women with very early (<40 years) or late (>55 years) menopause did not materially influence the results with age at menopause as a continuous variable. When these women were excluded from the analysis, the mean percentage of reduction in mortality associated with a 3-year increase in age at menopause was 1.7 percent (95 percent confidence interval: 0.4, 3.0). In the three different age groups (<70 years, 7079 years, and 80 years or above), the mean reductions in mortality were 3.2 percent (95 percent confidence interval: 1.4, 7.6), 2.8 percent (95 percent confidence interval: 0.3, 5.1), and 1.0 percent (95 percent confidence interval: 0.6, 2.7), respectively.
Table gives the results from the analyses within strata defined by a number of possible confounders and effect modifiers. The analyses confirm the results from the main analysis presented in table . No interactions were indicated, and the consistency of the weak inverse relation is striking, except possibly for occupational categories (p = 0.52 for heterogeneity). The association between age at menopause and all-cause mortality hardly differed over birth cohorts (p value for linear trend = 0.92). The data may suggest a stronger inverse relation in multiparous women than in those who had not given birth to a child, but no linear trend over categories defined by parity could be established (p = 0.44).
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In women with information about body mass index, we found a 1.0 percent (95 percent confidence interval: 0.3, 2.4) reduction in mortality with an increase in age at natural menopause of 3 years. Adjustment for body mass index (in addition to the standard adjustments) did not influence the association between age at menopause and total mortality (estimated reduction, 1.0 percent (95 percent confidence interval: 0.4, 2.3)).
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DISCUSSION |
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The number of deaths in our study is considerably larger than in earlier studies, 10 times that of the previously largest one, the last follow-up of Californian Seventh-day Adventists (2). The direction of the relation found by us is consistent with results in previous studies (2, 7, 8, 10), but the mortality rate ratio for women with late menopause (55 years) compared with women with premature menopause (<40 years) may be weaker than estimates found previously (2, 7, 10). One reason for this could be the very long follow-up (19611997) with the large majority of the deaths occurring in old age, when relations between age at menopause and total mortality may be attenuated. Other explanations of the weaker relation in our study should, however, also be considered. For example, the distribution of causes of death may differ between populations.
Our results are not conclusive with regard to whether the effect of age at natural menopause is attenuated with increasing age (1). Previous studies have suggested a stronger inverse relation in women who were relatively young at baseline, that is, in the first decades after menopause (3, 7). If an attenuation of the relation does in fact take place, this may simply reflect the longer time since a premenopausal state characterized by, inter alia, high levels of endogenous estrogen. With increasing age-related mortality, the relative importance of each risk factor may also be reduced. Although the difference in total mortality with increasing age at natural menopause is moderate, a reduction of 2.5 percent in the total mortality associated with 3 years increase in age at menopause in women aged less than 80 years cannot be considered negligible on a population level. The magnitude is comparable with the hypothetical effects of eradication of cancer of the ovaries as a cause of death, for example (accounting for 1.8 percent of the deaths before the age of 80 years in this cohort). For the individual woman, however, the reduction of the absolute mortality rate (six per 10,000 person-years) is probably of marginal importance.
Our analyses did not include women who were premenopausal at the time of screening. As women with an early menopause therefore were overrepresented in the young birth cohorts and as the total mortality rate was inversely related to birth cohort, we adjusted for birth cohort in all analyses, and we also carried out separate analyses restricted to women born before 1894 and during 18951904 to verify the overall results (table ).
The prospective design of our study makes biased reporting of age at menopause unlikely. However, there will be some misclassification of age at menopause when it is self-reported, and the misclassification may increase with the time since menopause (1416). In our study, the women had been postmenopausal on an average of approximately 10 years when they reported age at menopause. The misclassification of age at menopause must, however, be expected to be mostly unrelated to the age-adjusted mortality. In view of this misclassification, the inverse relation between age at natural menopause and total mortality may be stronger than we are able to demonstrate.
Many determinants have been suggested for age at menopause, including for example genetic factors (17, 18), childhood cognitive function (19), and even a hypothetical male (pheromonal) influence (20). Demographic variables (e.g., never been married and low social class), reproductive factors (e.g., late menarche, nulliparity, never used oral contraceptives), and behavioral variables (e.g., smoking and body mass index (both high and low)) have also been associated with early natural menopause (21, 22).
The inverse relation was a consistent finding in most of the subgroups of our material, thus suggesting that confounding by factors potentially affecting age at menopause was not a significant problem. This conclusion is also supported by results from our analyses with adjustment for body mass index and by analyses with adjustment for parity and age at menarche and at first delivery (as well as last delivery).
Smoking is the behavioral factor that has most consistently been found to be related to early menopause, as it lowers the age at menopause by 12 years (21, 23, 24) and increases death rates of several major causes such as coronary heart disease and cancer. Considering the relatively weak relation between age at menopause and total mortality and the impact of smoking on total mortality also in Norway (25), smoking may be suggested as a likely confounder.
Unfortunately, no information was available about smoking habits of the individual women in our study. However, a large majority of the women in the birth cohorts considered were nonsmokers, and the mean number of cigarettes per day in women who smoked was much lower than in men (26, 27). From the national data presented by Rønneberg et al. (26), we have estimated the smoking prevalence in Norwegian women aged 4549 years in the birth cohorts included. The age interval, 4549 years, was selected as the relevant age group because current, but not former, smoking is associated with earlier age at menopause (21, 22). The mean smoking prevalence was estimated at not more than 15 percent (26). Smoking prevalence among women in our cohort, living predominantly in rural parts of the country, was lower than in Norway in general (26, 27).
If our results were explained by smoking, we would expect a very weak or no relation in women born before 1895 because, in this group of women, the smoking prevalence when they were aged 4549 years was hardly higher than 5 percent and probably lower (26). As shown in table , the mean reduction in mortality associated with 3 years change in age at menopause was 1.6 percent (95 percent confidence interval: 0.3, 3.5 percent); that is, the point estimate was the same as in the complete data set. Furthermore, by adjusting for birth cohort and occupational group (and in some analyses for parity and age at first and last delivery), we have to some extent also adjusted for smoking, because birth cohort is particularly correlated with smoking prevalence. However, we recognize that there may be residual confounding by smoking in our analysis and that our overall risk estimates may therefore still be biased.
Hormone replacement therapy could have introduced both misclassification with regard to age at menopause and possible confounding, if there were any associations between age at natural menopause and use of hormone replacement therapy and if such therapy influenced total mortality. However, hormone replacement therapy was very uncommon for women in this cohort. We have excluded all women who reported surgery, such as uni- or bilateral oophorectomy, that may have been followed by prescription of estrogens. Hormone therapy for perimenopausal complaints (e.g., heat flashes) was very seldom used in Norway in the late 1950s and was uncommon during follow-up (1, 28). Ninety-nine percent of the women included in our analysis were born before 1912; that is, they were 50 years or older when estrogen medication for use in peri- and postmenopausal years was introduced in Norway. Thus, because of the birth year of the women, the prevalence of hormone replacement therapy in the women included in the analyses is without doubt very low, if not nearly zero. For similar reasons, the use of oral contraceptives does not represent a relevant confounder in our analysis.
A low age at natural menopause may in some women reflect clinical or subclinical medical conditions that may influence the mortality of the women. For example, a recent study demonstrated reduced age at natural menopause in women with diabetes mellitus type I (29). We have not made any effort to exclude these women from the analysis as we have studied the net effect of age at natural menopause on all-cause mortality. The hypothesis tested is that an early menopause is related to increased mortality, but it is clear that this hypothesis must be a crude simplification because it is well known that a low age at menopause reduces the risk of the most frequent cancer among Norwegian women, breast cancer (5, 6). The opposite is true for the risk of osteoporosis (30) (reflected in hip fracture mortality in our population (31)) and probably for coronary heart disease mortality (1, 2, 4). However, as coronary heart disease mortality rates are much higher than the mortality caused by breast cancer (32), the net result is an inverse relation. In order to get a more comprehensive picture of why an early menopause increases the mortality of some major causes of death and reduces the mortality of others, it may be necessary to study the determinants of age at natural menopause in more detail and to explore relations between age at menopause and cause-specific mortality. Nevertheless, establishing the relation between age at natural menopause and total mortality is a natural starting point for such an endeavor.
In summary, our very long follow-up study of 19,731 women with a natural menopause demonstrates an inverse relation of moderate strength between age at natural menopause and total mortality, and it suggests that this relation is stronger the younger the women are. The association was found consistently in stratified analysis and could not be explained by available information about possible confounders. Thus, women who experience an early natural menopause are subject to a slightly increased mortality.
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NOTES |
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REFERENCES |
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