From the Division of Cancer Epidemiology and Genetics, National Cancer Institute, Department of Health and Human Services, Bethesda, MD.
Received for publication December 1, 2003; accepted for publication March 16, 2004.
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ABSTRACT |
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carcinogens; cohort studies; formaldehyde; lung; mortality; nasopharynx; neoplasms; occupational health
Abbreviations: Abbreviations: CI, confidence interval; SMR, standardized mortality ratio.
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INTRODUCTION |
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In 1995, the International Agency for Research on Cancer found sufficient evidence for the carcinogenicity of formaldehyde in animals but only limited evidence for carcinogenicity in humans (2). Some studies of industrial workers or embalmers, pathologists, and anatomists have associated formaldehyde exposure with cancer of the nasal cavities (57), nasopharynx (5, 714), prostate (10, 15), lung (1618), pancreas (19), brain (10, 15, 2024), and lymphohematopoietic system (8, 10, 15, 17, 20, 23, 25, 26). However, these associations were inconsistent and remain controversial.
In this study, we assessed the relation between formaldehyde and selected solid cancers in an extended follow-up of the largest known cohort to date of industrial workers in formaldehyde industries. In a separate analysis of these data (27), we observed a significant association between mortality from leukemia, particularly myeloid leukemia, and peak and average exposure to formaldehyde.
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MATERIALS AND METHODS |
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On the basis of information from secondary sources other than death certificates, it was found that one of the nasopharyngeal cancer subjects had been misclassified on the death certificate and in fact had cancer of the tonsillar fossa (29). For this subject, nasopharyngeal cancer was used as the cause of death to calculate standardized mortality ratios, since population reference rates are based on death certificates, but cancer of the oropharynx, of which the tonsillar fossa is a part, was used to estimate relative risks.
Exposure assessment
We estimated exposure to formaldehyde from work histories through 1980 based on job titles, tasks, visits to the plants by study industrial hygienists, discussions with workers and plant managers, and monitoring data. Peak exposures were defined as short-term excursions (generally less than 15 minutes) that exceeded the 8-hour, time-weighted average formaldehyde intensity. Peak exposures in the workplace occurred from routine (e.g., hourly, daily, or weekly) or nonroutine performance of high-exposure tasks or from working in areas where nonroutine, unusual upsets or events, such as spills, occurred. Since no measurements of peak exposure were available in this study, peaks and their frequency (hourly, daily, weekly, or monthly) were estimated by an industrial hygienist from knowledge of the job tasks and a comparison with the 8-hour time-weighted average. We assessed the presence of particulates to represent formaldehyde as a solid (e.g., paraformaldehyde or trioxane), formaldehyde-containing resins, molding compound particulates, or particulates onto which formaldehyde gas could be adsorbed. Exposures to 11 suspected carcinogens and other widely used chemicals in the plants were evaluated (antioxidants, asbestos, carbon black, dyes and pigments, hexamethylenetetramine, melamine, phenol, plasticizers, urea, wood dust, and benzene). We also identified workers employed as chemists or laboratory technicians because of their potential exposure to various other chemicals. The exposure assessment is described in detail elsewhere (5, 30, 31). For the extended follow-up, no information on formaldehyde exposure after 1980 was obtained.
Statistical analysis
The following formaldehyde exposure metrics were calculated as time-dependent variables: cumulative exposure (ppm-years), average exposure intensity (ppm), duration of exposure (years), highest peak exposure category (nonexposed, >0<0.5 ppm, 0.5<2.0 ppm, 2.0<4.0 ppm, 4.0 ppm), exposure to formaldehyde-containing particulates (ever/never), duration of exposure to each of 11 other substances (years), and duration of working as a chemist or laboratory technician (years). Workers contributed person-time to the nonexposed category until they were exposed. Then, they contributed person-time to the appropriate exposure categories depending on their levels of exposure. For each worker, we collapsed jobs in adjacent time periods of exposure for which all of the estimated exposure variables were identical. However, for the description of exposure levels in these jobs, nonadjacent periods with identical estimated exposures were counted separately.
Standardized mortality ratios and relative risks were estimated by using standard methods (32). Relative risks were based on Poisson regression models and were adjusted for calendar year, age, sex, race, and pay category (32). The low-exposure category was used as the reference to minimize the impact of any unmeasured confounding variables, since nonexposed workers may differ from exposed workers with respect to socioeconomic characteristics. However, workers in the low-exposure category were exposed to very low levels of formaldehyde. We evaluated confounding for exposure to other substances and for working as a chemist or laboratory technician. Tests of trend for categorical variables were based on the estimated slope of the corresponding continuous variable, except for peak exposure, where categorical ranks were used. Heterogeneity among risk estimates was assessed by likelihood ratio tests. Tests were two-sided at a 5 percent significance level. For details of the statistical analysis, refer to the separate report by Hauptmann et al. (27).
We calculated all exposures by using a 15-year lag interval to account for latency of solid cancers. Lag intervals from 2 to 20 years were evaluated, but no substantial differences for model fit from the 15-year lag were found for solid cancers of primary interest. The 15-year lag interval was eventually chosen because 15 years is commonly regarded as a minimum latency time for solid tumors and because it conforms to the lack of exposure information for the extension of the follow-up (19801994).
Since plant was correlated with exposure, we do not present relative risk estimates adjusted for plant in this paper. However, adjusting for plant did not substantially change the results.
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RESULTS |
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Cancer mortality and exposure to formaldehyde
Compared with that for the US population, mortality from solid cancers was significantly decreased in nonexposed (standardized mortality ratio (SMR) = 0.78) and exposed (SMR = 0.91) workers (table 2). Significant deficits occurred for cancers of the digestive system, pancreas, lung, bone, and prostate in the nonexposed and for cancers of the digestive system, breast, and bladder in the exposed. Excesses among exposed workers were observed for cancers of the nasopharynx, nose and nasal cavity, and bone. On the basis of the relative risks, no consistent evidence of increasing risks was found for mortality from all solid cancers combined with any measure of formaldehyde exposure (tables 3, 4, 5, and 6).
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Other cancer sites of a priori interest were the pancreas, prostate, and brain. Mortality from cancer of the pancreas was not associated with any of our measures of formaldehyde exposure in the total cohort (tables 3, 4, 5, and 6). Relative risks increased with average exposure intensity only for the subgroup of older subjects (aged 65 years), but the trend was not statistically significant (table 7). For prostate cancer, a significantly elevated relative risk of 1.61 (95 percent CI: 1.04, 2.47) occurred for workers with peak formaldehyde exposure of 2.0<4.0 ppm (42 deaths), but the trend with peak exposure was not statistically significant (table 4). Relative risks for categories of average exposure intensity were slightly elevated, and the trend was borderline significant (p = 0.065) for exposed subjects (table 3) and significant or borderline significant for the subgroups of White workers (p = 0.053), older workers (aged
65 years, p = 0.086), wage workers (p = 0.067), and workers never exposed to formaldehyde-containing particulates (p = 0.021) (table 7). No association was observed for mortality from malignant brain tumors (62 deaths) (tables 3, 4, 5, and 6).
Some associations were observed for cancers not of a priori interest. There were seven deaths from bone cancer among exposed workers (SMR = 1.57 among exposed) and none among nonexposed workers (2.9 expected). Relative risks increased with exposure, particularly for cumulative exposure. The relative risks for workers exposed for 1.5<5.5 and 5.5 ppm-years were 1.91 (95 percent CI: 0.31, 11.64) and 2.53 (95 percent CI: 0.40, 16.03), respectively, compared with workers exposed to low levels (>0<1.5 ppm-years) of formaldehyde, with a significant trend for exposed workers (p = 0.032) (table 5). For liver cancer (31 deaths), we found an association with peak exposure. When we compared workers exposed to peak levels of 2.0<4.0 ppm and
4.0 ppm with workers exposed to low peak levels of formaldehyde (>0<2.0 ppm), the relative risks were 1.54 (95 percent CI: 0.50, 4.73) and 2.18 (95 percent CI: 0.80, 5.99), respectively, with a significant trend for exposed workers (p = 0.045) (table 4). However, no association was observed for other exposure measures (tables 3, 5, and 6).
Exposure to substances other than formaldehyde
Forty-seven percent of the subjects were ever occupationally exposed to at least one of the following substances: antioxidants (22 percent), asbestos (14 percent), carbon black (11 percent), dyes and pigments (16 percent), hexamethylenetetramine (15 percent), melamine (28 percent), phenol (14 percent), plasticizers (20 percent), urea (27 percent), wood dust (10 percent), and benzene (2 percent). Relative risks for various cancers and formaldehyde exposure categories did not change substantially when adjusted for duration of exposure to these substances, except for nasopharyngeal cancer and melamine exposure. For that site, relative risks for the highest exposure categories of peak and average intensity of formaldehyde exposure declined when the analysis was adjusted for melamine exposure (data not shown). However, relative risks were still elevated for cumulative exposure and duration of exposure after adjustment for melamine exposure, and trend tests remained significant for peak (p < 0.001), average (p = 0.021), and cumulative (p = 0.006) exposure. We repeated the analyses for all cancers of interest by excluding the 586 subjects exposed to benzene and found no substantial differences. Only 8 percent of all workers were employed as chemists or laboratory technicians, and only 2 percent worked in such jobs for 5 or more years. Adjusting for duration of working as a chemist or laboratory technician did not substantially change the observed associations.
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DISCUSSION |
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Risk estimates for lung cancer from formaldehyde exposure could have been confounded by other occupational exposures and smoking. Confounding from exposure to 11 other substances is less likely since there was no evidence of an association between lung cancer mortality and these exposures, and adjusting the analysis for duration of exposure to these 11 substances did not change the results. We lacked information on tobacco use for most of the cohort, but evidence suggests that smoking is not a confounder since there was no consistent excess or deficit for other tobacco-related diseases, for example, bladder cancer, emphysema, and ischemic heart disease. Information on smoking habits obtained from medical records for a small sample of workers from two plants (63 subjects with cancer and 316 age-matched controls) revealed no major differences in smoking prevalence by level of cumulative formaldehyde exposure (28). Pay category, which correlates with socioeconomic status and smoking prevalence, was included as an adjustment factor in the analysis. Our null finding for formaldehyde exposure and lung cancer is consistent with several recent studies (26, 3941), although other studies of industrial populations have suggested increased lung cancer mortality (1618).
The factor plant was taken into account in our analysis. We directly addressed potential confounding by plant-related factors by adjusting for 11 potentially confounding substances. Directly adjusting for plant may result in overadjustment. However, to address the potential effect of unmeasured confounders associated with plant, we performed analyses adjusted for plant. Although some of these analyses were based on small numbers, and, as a consequence, estimates had large variances, associations observed for cancers of the upper respiratory tract, nasopharynx, salivary gland, nose or nasal cavity, and bone remained after we adjusted for plant. In the adjusted analysis, no clear association was seen for cancers of the pancreas, brain, lung, or prostate.
Inhaled formaldehyde is deposited almost entirely in the upper respiratory tract of rats (42) and is rapidly incorporated into DNA, RNA, and proteins (43). Therefore, the upper respiratory tract is the site of direct exposure for inhaled formaldehyde. Despite the small numbers of deaths from cancers of the upper respiratory tract, the positive association for this site as a group with average intensity and peak exposure in our analysis is consistent with the carcinogenicity of formaldehyde at the site of first contact. Several epidemiologic (712, 14, 24, 44) and animal (3, 4) studies support these results for specific sites in the upper respiratory tract, while other studies (16, 20, 26, 39, 4547) provide little support.
Some cohort studies (10), including ours, and some case-control studies (9, 1114) reported a positive association between formaldehyde exposure and nasopharyngeal cancer, whereas others (16, 20, 26, 39, 4547) did not. One study found a positive association for hypopharyngeal cancer (48). The excess for nasopharyngeal cancer reported previously in this cohort persisted, although only three additional deaths occurred in the extended follow-up. We observed exposure-response patterns for nasopharyngeal cancer for average, cumulative, and peak exposure to formaldehyde. Because five of the nine deaths from nasopharyngeal cancer occurred at one plant (plant 1; table 1), we performed analyses adjusted for plant and found increasing relative risks with increasing exposure categories for all four exposure metrics. Specifically, adjusted relative risks for the categories shown in tables 3, 4, 5, and 6 were, respectively, 1.00, (not applicable), (not applicable), and 9.07 for peak exposure (p-trend among exposed = 0.008); 1.00, (not applicable), 8.51, and 23.54 for average intensity (p-trend among exposed = 0.404); 2.18, 1.00, 1.34, and 5.32 for cumulative exposure (p-trend among exposed = 0.007); and 1.76, 1.00, 1.21, and 8.59 for duration of exposure (p-trend among exposed = 0.043). These results are consistent with increasing standardized mortality ratios with increasing cumulative exposure and duration of exposure to formaldehyde found in an independent investigation of workers at this plant (including all workers hired between 1941 and 1984 and followed through 1998) (49).
Of the nine workers who died from nasopharyngeal cancer, two were not exposed to formaldehyde and were never exposed to particulates, whereas seven workers were exposed to formaldehyde and to particulates. This complete colinearity of exposure to formaldehyde and particulates prevented us from evaluating formaldehyde exposure separately for those workers exposed and not exposed to particulates. However, nasopharyngeal cancer risk increased with formaldehyde exposure for those exposed to particulates, and formaldehyde intensities and peak exposures ranged from low to high in the jobs involving and not involving particulate exposure held by the nine workers who died from nasopharyngeal cancer. This finding provides evidence that the association seen for formaldehyde may not be entirely due to particulates.
Workers could contribute person-time and deaths to high peak exposure categories based on infrequent peaks because peaks may have occurred in jobs of short duration or occurred less often than daily or weekly, or both. We created several alternative maximum peak exposure metrics, ignoring peaks in jobs of short duration (<6 or <12 months) or rare peaks (less often than daily or weekly), and found two- to sevenfold increased risks for nasopharyngeal cancer in the highest peak exposure category (4.0 ppm) compared with the nonexposed category.
Wood dust is a potential confounder for formaldehyde exposure and nasal and nasopharyngeal cancer (2, 47, 50); however, none of our nasal and nasopharyngeal cancer cases had been identified as being exposed to wood dust. For nasopharyngeal cancer, some confounding was observed by duration of exposure to melamine. Exposure to melamine occurred at six plants, mainly in the manufacture of synthetic resins with formaldehyde. Although exposure to high doses of melamine produced urinary bladder and ureteral carcinomas in rats, there is inadequate evidence for the carcinogenicity of melamine in humans (51). Therefore, the observed association between melamine exposure and nasopharyngeal cancer and subsequent confounding of the formaldehyde-nasopharyngeal cancer association may be spurious. No information was available on the presence of antibodies to Epstein-Barr virus, another major risk factor for nasopharyngeal cancer (52).
Inhaled formaldehyde causes nasal cavity tumors in mice (3), and some epidemiologic studies have reported a positive association between formaldehyde exposure and cancer of the nasal cavity (44, 53, 54). A meta-analysis found an increased risk in 11 case-control studies but not in nine cohort studies of industrial workers (39), although many of the studies that did not show an association had generally low power because of small numbers of cases, uncertainties in the exposure assessment, or both. The association for cancer of the nasal cavity found in the current analysis is consistent with an effect, but the number of deaths was too limited to enable a firm conclusion.
In a cohort of workers exposed to formaldehyde in the garment industry, Stayner et al. (55) found a significant excess of cancer of the buccal cavity (3 observed, 0.4 expected), with the three observed deaths attributed to cancer of the parotid gland, which is part of the salivary gland. Although our numbers were also small regarding cancer of the salivary gland (four deaths), we did see increasing relative risks with categories of cumulative exposure and duration of exposure. This finding is consistent with recent data from a death-certificate-based case-control study including 2,405 salivary gland cancer deaths and showing an increased risk with occupational exposure to formaldehyde (56).
Our finding of no association between formaldehyde exposure and pancreatic cancer is consistent with a recent review and meta-analysis of 14 studies; no elevated risk was found for industrial workers, although a slightly elevated risk was found for embalmers, pathologists, and anatomists (19).
The association between formaldehyde exposure and prostate cancer has been mixed, with weakly positive associations (10, 15), no associations (16, 20, 21), and protective effects (24, 45) reported. In the initial report on this cohort (5), a slight excess was confined largely to salaried workers, suggesting that the association was due to socioeconomic factors rather than occupational exposures. The moderate positive association between formaldehyde exposure and prostate cancer observed in the current analysis, especially for wage workers and older workers, is suggestive, but the absence of a clear exposure-response gradient and internal inconsistencies among wage and salaried workers do not provide much evidence for a causal relation.
Most studies of embalmers or pathologists have reported nonsignificantly elevated standardized mortality ratios for brain cancer (10, 15, 2022). One study of anatomists found significantly elevated standardized mortality ratios that increased with duration of membership in the anatomists association (23). For industrial workers, no association (6, 16, 26, 45, 46) or small excesses (24) have been reported. The previous analysis of this cohort (5) found no link between brain cancer and formaldehyde exposure, and we found no association after the extended follow-up.
The excess mortality from bone cancer is interesting, but, to our knowledge, this site has not been linked with formaldehyde exposure in previous experimental or epidemiologic investigations. Interpretation of the finding is problematic because of the small number of deaths (n = 7) and because the bone is a common site of metastases. However, the size of the relative risk and occurrence among only the exposed suggest that further consideration is warranted. As far as we know, liver cancer has not been linked to formaldehyde exposure, and the observed association may be a chance finding.
Our study has limitations. Extension of mortality follow-up from 1980 through 1994 utilized only the National Death Index Plus to determine vital status. Subjects not identified as deceased by this source were assumed to be alive. Although the National Death Index Plus is quite complete, it is possible that there was some underascertainment of deaths. However, it is unlikely that this factor would bias relative risk estimates because missing deaths are unlikely to be related to formaldehyde exposure. Exposure misclassification is always a concern in epidemiologic investigations. The detailed quantitative assessment of time-weighted average exposure intensity in this study used monitoring data provided by the companies, monitoring in each plant by study investigators (33), visits to the plants by study industrial hygienists, and discussions with plant managers and long-time workers (30). Therefore, this process should minimize misclassification for average and cumulative exposure and duration of exposure. Assessment of peak exposure could have been more susceptible to misclassification since peak levels were estimated from job tasks and the time-weighted average exposure. However, since any misclassification of formaldehyde exposure most likely was nondifferential, the potential effect would be an attenuation of risk estimates. Therefore, exposure misclassification could explain a lack of association, but the exposure assessment procedure was sufficient to yield an exposure-response relation with nasopharyngeal cancer and leukemia (27), lending support to the null findings for lung cancer and other a priori sites.
The study also has a number of strengths. Follow-up was as long as 60 years, and there was extensive information on formaldehyde exposure. The long follow-up yielded 8,486 deaths, which provided adequate power to detect relatively small effects for common cancer sites. We had at least 80 percent power to detect a 1.3-fold lung cancer relative risk for workers exposed to high versus low levels of formaldehyde for cumulative exposure, peak exposure, and average intensity. We were able to assess formaldehyde exposure according to several measures that characterize different aspects of exposure, thereby diminishing the chances that a true association was missed because an inappropriate exposure metric was chosen. Biases from exposure misclassification, confounding, or other factors may have influenced results for one exposure measure but are less likely to have affected all measures equally (57), thus allowing for a more robust interpretation of the data. We were able to control for possible confounding from a number of other workplace chemicals. Availability of information on tobacco use for a small subset of workers indicates that smoking was not related to formaldehyde exposure and thus should not have been a confounder. In addition, we did not rely on external comparisons (SMRs), which are subject to a healthy worker bias (58), but instead focused on internal analyses comparing similar subjects.
In summary, analysis of this cohort of workers in the formaldehyde industry, which included additional years of follow-up, supports a possible causal association with mortality from cancer of the nasopharynx and possibly other upper respiratory tract sites. The association with prostate cancer could be a chance finding since there was no exposure-response gradient. Because bone is a common metastatic site, the observed excess of bone cancer is difficult to interpret. No association was seen with cancers of the pancreas, the brain, or the lung.
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ACKNOWLEDGMENTS |
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NOTES |
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REFERENCES |
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